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Fast electrophoresis in conventional capillaries by employing a rapid injection device and contactless conductivity detection

Rainelli, Andrea ; Hauser, Peter

In: Analytical and Bioanalytical Chemistry, 2005, vol. 382, no. 3, p. 789-794

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    Titre
    • Fast electrophoresis in conventional capillaries by employing a rapid injection device and contactless conductivity detection
    Auteur
    • Rainelli, Andrea. Department of Chemistry, University of Basel, Spitalstrasse 51, 4004, Basel, Switzerland
    • Hauser, Peter. Department of Chemistry, University of Basel, Spitalstrasse 51, 4004, Basel, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Analytical and Bioanalytical Chemistry, 2005, vol. 382, no. 3, p. 789-794. Springer-Verlag; http://www.springer.de
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:320495
    Summary
    A purpose-made set-up featuring an automated fast injector allowed the easy optimization of the injected amount and the adjustment of the separation length of conventional capillaries from a minimum of 5cm upward. It was found that a compromise in capillary length for separation efficiency and analysis time also has to take into account the injected amount, which in turn affects the sensitivity and hence the detection limit. The versatility of the system was demonstrated by the analysis of the major cations and anions in natural water samples in less than 1min, the concurrent determination of a mixture of amino acids and carbohydrates in 160s, and of three active ingredients in a pharmaceutical preparation in 40s. Plate numbers were typically around 50,000 and detection limits down to 1μM could be achieved