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The Combination of Electric Current and Copper Promotes Neuronal Differentiation of Adipose-Derived Stem Cells

Jaatinen, L. ; Salemi, S. ; Miettinen, S. ; Hyttinen, J. ; Eberli, D.

In: Annals of Biomedical Engineering, 2015, vol. 43, no. 4, p. 1014-1023

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    Titre
    • The Combination of Electric Current and Copper Promotes Neuronal Differentiation of Adipose-Derived Stem Cells
    Auteur
    • Jaatinen, L.. Department of Electronics and Communications Engineering, Tampere University of Technology and BioMediTech, Tampere, Finland
    • Salemi, S.. Urologic Tissue Engineering and Stem Cell Therapy, Department of Urology, University Hospital Zürich, Frauenklinikstr. 10, 8091, Zurich, Switzerland
    • Miettinen, S.. Adult Stem Cells, Institute of Biomedical Technology, University of Tampere, Tampere, Finland
    • Hyttinen, J.. Department of Electronics and Communications Engineering, Tampere University of Technology and BioMediTech, Tampere, Finland
    • Eberli, D.. Urologic Tissue Engineering and Stem Cell Therapy, Department of Urology, University Hospital Zürich, Frauenklinikstr. 10, 8091, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Annals of Biomedical Engineering, 2015, vol. 43, no. 4, p. 1014-1023. Springer US; http://www.springer-ny.com
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:331653
    Summary
    Damage to the nervous system can be caused by several types of insults, and it always has a great effect on the life of an individual. Due to the limited availability of neural transplants, alternative approaches for neural regeneration must be developed. Stem cells have a great potential to support neuronal regeneration. Human adipose-derived stem cells (hADSCs) have gained increasing interest in the fields of regenerative medicine due to their multilineage potential and easy harvest compared to other stem cells. In this study, we present a growth factor-free method for the differentiation of hADSCs toward neuron-like cells. We investigated the effect of electric current and copper on neuronal differentiation. We analyzed the morphological changes, the mRNA and protein expression levels in the stimulated cells and showed that the combination of current and copper induces stem cell differentiation toward the neuronal lineage with elongation of the cells and the upregulation of neuron-specific genes and proteins. The induction of the neuronal differentiation of hADSCs by electric field and copper may offer a novel approach for stem cell differentiation and may be a useful tool for safe stem cell-based therapeutic applications.