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Concanavalin A electrochemical sensor based on the surface blocking principle at an ion-selective polymeric membrane

Pawlak, Marcin ; Mistlberger, Günter ; Bakker, Eric

In: Microchimica Acta, 2015, vol. 182, no. 1-2, p. 129-137

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    Title
    • Concanavalin A electrochemical sensor based on the surface blocking principle at an ion-selective polymeric membrane
    Author
    • Pawlak, Marcin. Department of Inorganic and Analytical Chemistry, University of Geneva, Quai E.-Ansermet 30, 1211, Geneva, Switzerland
    • Mistlberger, Günter. Department of Inorganic and Analytical Chemistry, University of Geneva, Quai E.-Ansermet 30, 1211, Geneva, Switzerland
    • Bakker, Eric. Department of Inorganic and Analytical Chemistry, University of Geneva, Quai E.-Ansermet 30, 1211, Geneva, Switzerland
    Document Type
    • Postprint
    OAI-PMH Identifier
    • oai:doc.rero.ch:331053
    Summary
    We report on a new electrochemical sensor for Concanavalin A. It is based on blocking the surface of plasticized PVC membranes that were covalently modified with D-mannose using click chemistry. The interaction of D-mannose with Concanavalin A on the surface perturbs the flux of a marker ion for which the ion-selective membrane is responsive, and this results in a change in the electrochemical signal. The sensor was characterized using a variety of electrochemical protocols, and results were confirmed by quartz crystal microbalance experiments. The lowest limit of detection (10μgmL−1) was obtained using a membrane containing a cation exchanger and tetrabutylammonium ion as the marker ion. Figure An electrochemical sensor for Concanavalin A based on the surface blocking principle was prepared by covalent modification of a plasticized PVC membrane surface with D-mannose by click chemistry