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The sensitivity of an interferon-γ release assay in microbiologically confirmed pediatric tuberculosis

Schopfer, Kurt ; Rieder, Hans ; Bodmer, Thomas ; Steinlin-Schopfer, Jacqueline ; Chantana, Yay ; Studer, Peter ; Laurent, Denis ; Richner, Beat

In: European Journal of Pediatrics, 2014, vol. 173, no. 3, p. 331-336

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    Titre
    • The sensitivity of an interferon-γ release assay in microbiologically confirmed pediatric tuberculosis
    Auteur
    • Schopfer, Kurt. Institute of Infectious Diseases, University of Bern, Friedbühlstrasse 51, 3010, Bern, Switzerland
    • Rieder, Hans. International Union Against Tuberculosis and Lung Disease, 68 blvd Saint-Michel, 75006, Paris, France
    • Bodmer, Thomas. Institute of Infectious Diseases, University of Bern, Friedbühlstrasse 51, 3010, Bern, Switzerland
    • Steinlin-Schopfer, Jacqueline. Institute of Infectious Diseases, University of Bern, Friedbühlstrasse 51, 3010, Bern, Switzerland
    • Chantana, Yay. Kantha Bopha Foundation, Phnom Penh, Cambodia, c/o Intercontrol AG, Seefeldstrasse 17, 8008, Zurich, Switzerland
    • Studer, Peter. Kantha Bopha Foundation, Phnom Penh, Cambodia, c/o Intercontrol AG, Seefeldstrasse 17, 8008, Zurich, Switzerland
    • Laurent, Denis. Kantha Bopha Foundation, Phnom Penh, Cambodia, c/o Intercontrol AG, Seefeldstrasse 17, 8008, Zurich, Switzerland
    • Richner, Beat. Kantha Bopha Foundation, Phnom Penh, Cambodia, c/o Intercontrol AG, Seefeldstrasse 17, 8008, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • European Journal of Pediatrics, 2014, vol. 173, no. 3, p. 331-336. Springer Berlin Heidelberg
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:324891
    Summary
    This study aimed at determining the sensitivity of a whole blood interferon-γ release assay (IGRA) among children with microbiologically confirmed tuberculosis in a high-burden country. Children with a diagnosis of tuberculosis based on clinical and radiographic assessment were tested with an IGRA in addition to microbiologic examination of appropriate specimens for acid-fast bacilli, mycobacterial rRNA, and observation for growth of Mycobacterium tuberculosis on appropriate culture media. Of the 405 children with a clinical diagnosis of tuberculosis, 91 (22.5%) had microbiologically confirmed tuberculosis, of whom 81 were tested with an IGRA. A positive result was obtained in 43 (sensitivity 53.1%, 95% confidence interval 42.3 to 63.6%), uninfluenced by age, sex, or disease manifestation. Conclusions: The sensitivity of a whole blood interferon-γ release assay in microbiologically confirmed pediatric tuberculosis was low. An IGRA cannot, thus, be used as rule-in test, but it might be useful to rule in tuberculosis among children in whom tuberculosis is notoriously difficult to confirm microbiologically.