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The metal-binding properties of the blue crab copper specific CuMT-2: a crustacean metallothionein with two cysteine triplets

Serra-Batiste, Montserrat ; Cols, Neus ; Alcaraz, Luis ; Donaire, Antonio ; González-Duarte, Pilar ; Vašák, Milan

In: JBIC Journal of Biological Inorganic Chemistry, 2010, vol. 15, no. 5, p. 759-776

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    Titel
    • The metal-binding properties of the blue crab copper specific CuMT-2: a crustacean metallothionein with two cysteine triplets
    Autor
    • Serra-Batiste, Montserrat. Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain
    • Cols, Neus. Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 645, 08028, Barcelona, Spain
    • Alcaraz, Luis. Departamento de Química Física, Facultad de Ciencias, Universidad de Alicante, 03080, Alicante, Spain
    • Donaire, Antonio. Departamento de Química Inorgánica, Facultad de Química, Universidad de Murcia, 30100, Murcia, Spain
    • González-Duarte, Pilar. Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain
    • Vašák, Milan. Department of Biochemistry, University of Zurich, 8057, Zurich, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • JBIC Journal of Biological Inorganic Chemistry, 2010, vol. 15, no. 5, p. 759-776. Springer-Verlag
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:320774
    Summary
    Most crustacean metallothioneins (MTs) contain 18 Cys residues and bind six divalent metal ions. The copper-specific CuMT-2 (MTC) of the blue crab Callinectes sapidus with 21Cys residues, of which six are organized in two uncommon Cys-Cys-Cys sequences, represents an exception. However, its metal-binding properties are unknown. By spectroscopic and spectrometric techniques we show that all 21 Cys residues of recombinant MTC participate in the binding of Cu(I), Zn(II), and Cd(II) ions, indicating that both Cys triplets act as ligands. The fully metallated M8 II-MTC (MisZn, Cd) form possesses high- and low-affinity metal binding sites, as evidenced by the formation of Zn6-MTC and Cd7-MTC species from M8 II-MTC after treatment with Chelex 100. The NMR characterization of Cd7-MTC suggests the presence of a two-domain structure, each domain containing one Cys triplet and encompassing either the three-metal or the four-metal thiolate cluster. Whereas the metal-Cys connectivities in the three-metal cluster located in the N-terminal domain (residues 1-31) reveal a Cd3Cys9 cyclohexane-like structure, the presence of dynamic processes in the C-terminal domain (residues 32-64) precluded the determination of the organization of the four-metal cluster. Absorption and circular dichroism features accompanying the stepwise binding of Cu(I) to MTC suggest that all 21Cys are involved in the binding of eight to nine Cu(I) ions (Cu8-9-MTC). The subsequent generation of Cu12-MTC involves structural changes consistent with a decrease in the Cu(I) coordination number. Overall, the metal-binding properties of MTC reported here contribute to a better understanding of the role of Cys triplets in MTs