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Mitochondrial DNA content in paired normal and cancerous breast tissue samples from patients with breast cancer

Fan, Alex ; Radpour, Ramin ; Haghighi, Mahdi ; Kohler, Corina ; Xia, Peng ; Hahn, Sinuhe ; Holzgreve, Wolfgang ; Zhong, Xiao

In: Journal of Cancer Research and Clinical Oncology, 2009, vol. 135, no. 8, p. 983-989

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    Titre
    • Mitochondrial DNA content in paired normal and cancerous breast tissue samples from patients with breast cancer
    Auteur
    • Fan, Alex. Laboratory for Prenatal Medicine and Gynecologic Oncology, Department of Biomedicine, Women's Hospital, University of Basel, Hebelstrasse 20, Room Nr. 416, 4031, Basel, Switzerland
    • Radpour, Ramin. Laboratory for Prenatal Medicine and Gynecologic Oncology, Department of Biomedicine, Women's Hospital, University of Basel, Hebelstrasse 20, Room Nr. 416, 4031, Basel, Switzerland
    • Haghighi, Mahdi. Azad University, East of Tehran Branch, Tehran, Iran
    • Kohler, Corina. Laboratory for Prenatal Medicine and Gynecologic Oncology, Department of Biomedicine, Women's Hospital, University of Basel, Hebelstrasse 20, Room Nr. 416, 4031, Basel, Switzerland
    • Xia, Peng. Laboratory for Prenatal Medicine and Gynecologic Oncology, Department of Biomedicine, Women's Hospital, University of Basel, Hebelstrasse 20, Room Nr. 416, 4031, Basel, Switzerland
    • Hahn, Sinuhe. Laboratory for Prenatal Medicine and Gynecologic Oncology, Department of Biomedicine, Women's Hospital, University of Basel, Hebelstrasse 20, Room Nr. 416, 4031, Basel, Switzerland
    • Holzgreve, Wolfgang. Laboratory for Prenatal Medicine and Gynecologic Oncology, Department of Biomedicine, Women's Hospital, University of Basel, Hebelstrasse 20, Room Nr. 416, 4031, Basel, Switzerland
    • Zhong, Xiao. Laboratory for Prenatal Medicine and Gynecologic Oncology, Department of Biomedicine, Women's Hospital, University of Basel, Hebelstrasse 20, Room Nr. 416, 4031, Basel, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Journal of Cancer Research and Clinical Oncology, 2009, vol. 135, no. 8, p. 983-989. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:320552
    Summary
    Introduction: We develop a multiplex quantitative real-time PCR for synchronized analysis of mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) to investigate relative mtDNA abundance in paired normal and cancerous breast tissues. Materials and methods: The amounts of nDNA and mtDNA in 102 tissue samples were quantified for both glyceraldehype-3-phosphodehydrogenase (GAPDH) gene and mtDNA encoded ATPase (MTATP) 8 gene. The average threshold cycle (Ct) number values of the nDNA and mtDNA were used to calculate relative mtDNA content in breast tissues. Results: The median delta Ct (ΔCt) and the median mtDNA content for normal and cancerous breast tissues were 6.73 and 2.54, as well as 106.50 and 5.80 (P=0.000, respectively). The mtDNA content was decreased in 82% of cancerous breast tissues compared with the normal ones. The changes were associated with hormone receptor status. Conclusion: Our finding suggests that decreased mtDNA content in breast cancer may have diagnostic and prognostic value for the disease