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Muscle transcriptome adaptations with mild eccentric ergometer exercise

Klossner, Stephan ; Däpp, Christoph ; Schmutz, Silvia ; Vogt, Michael ; Hoppeler, Hans ; Flück, Martin

In: Pflügers Archiv - European Journal of Physiology, 2007, vol. 455, no. 3, p. 555-562

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    Titre
    • Muscle transcriptome adaptations with mild eccentric ergometer exercise
    Auteur
    • Klossner, Stephan. Institute of Anatomy, University of Berne, Berne, Switzerland
    • Däpp, Christoph. Institute of Anatomy, University of Berne, Berne, Switzerland
    • Schmutz, Silvia. Institute of Anatomy, University of Berne, Berne, Switzerland
    • Vogt, Michael. Institute of Anatomy, University of Berne, Berne, Switzerland
    • Hoppeler, Hans. Institute of Anatomy, University of Berne, Berne, Switzerland
    • Flück, Martin. Institute of Anatomy, University of Berne, Berne, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Pflügers Archiv - European Journal of Physiology, 2007, vol. 455, no. 3, p. 555-562. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:319500
    Summary
    The muscle has a wide range of possibilities to adapt its phenotype. Repetitive submaximal concentric exercise (i.e., shortening contractions) mainly leads to adaptations of muscle oxidative metabolism and endurance while eccentric exercise (i.e., lengthening contractions) results in muscle growth and gain of muscle strength. Modified gene expression is believed to mediate these exercise-specific muscle adjustments. In the present study, early alterations of the gene expression signature were monitored by a muscle-specific microarray. Transcript profiling was performed on muscle biopsies of vastus lateralis obtained from six male subjects before and in a 24-h time course after a single bout of mild eccentric ergometer exercise. The eccentric exercise consisted of 15min of eccentric cycling at 50% of the individual maximal concentric power output leading to muscle soreness (5.9 on a 0-10 visual analogue scale) and limited muscle damage (1.7-fold elevated creatine kinase activity). Muscle impairment was highlighted by a transient reduction in jumping height after the eccentric exercise. On the gene expression level, we observed a general early downregulation of detected transcripts, followed by a slow recovery close to the control values within the first 24h post exercise. Only very few regulatory factors were increased. This expression signature is different from the signature of a previously published metabolic response after an intensive endurance-type concentric exercise as well as after maximal eccentric exercise. This is the first description of the time course of changes in gene expression as a consequence of a mild eccentric stimulus