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Genetic diversity of Swiss maize ( Zea mays L. ssp. mays ) assessed with individuals and bulks on agarose gels

Eschholz, Tobias ; Peter, Roland ; Stamp, Peter ; Hund, Andreas

In: Genetic Resources and Crop Evolution, 2008, vol. 55, no. 7, p. 971-983

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    Titre
    • Genetic diversity of Swiss maize ( Zea mays L. ssp. mays ) assessed with individuals and bulks on agarose gels
    Auteur
    • Eschholz, Tobias. Pioneer Hibred Northern Europe, Muenstertaelerstr. 26, 79427, Eschbach, Germany
    • Peter, Roland. Institute of Plant Sciences, ETH Zurich, Universitaetstr. 2, 8092, Zurich, Switzerland
    • Stamp, Peter. Institute of Plant Sciences, ETH Zurich, Universitaetstr. 2, 8092, Zurich, Switzerland
    • Hund, Andreas. Institute of Plant Sciences, ETH Zurich, Universitaetstr. 2, 8092, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Genetic Resources and Crop Evolution, 2008, vol. 55, no. 7, p. 971-983. Springer Netherlands
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:318757
    Summary
    About 65years ago, more than 150 Swiss maize landraces (Zea mays L. ssp. mays) of the flint type were collected and conserved ex situ. Due to the climatically and culturally diverse environment of the Alps, a considerable genetic diversity of this material was assumed. To prove this, an efficient method was required to carry out genetic profiling of all the accessions in the Swiss Gene Bank. Simple sequence repeat marker (SSR) profiling in combination with the visualization of the polymerase chain reaction (PCR) products on agarose gels was chosen. Here a set of 19 different landrace accessions was analyzed to: (i) investigate their genetic diversity, (ii) investigate and display the population structure and (iii) determine whether DNA bulks rather than single plants can be used for such analyses. Four repeated samples of one accession were found to be much closer to one another than to the rest of accessions. Furthermore, specific alleles were identified for several accessions. The PCR products of the bulked DNA samples represented only a small part of the variation revealed by the analysis of individuals. Loci with four base repeat motifs performed better in the analysis of bulks than loci with other repeat motifs. The correlation between genetic distance matrices, based on the analysis of individuals and bulks, respectively, was significant. Thus, the single plant approach allowed for sufficient differentiation of accessions, and DNA bulks visualized on agarose gels led to correlated genetic distances although a limited number of alleles were detected. Although the limited resolution of agarose gels likely causes some bias, profiling of larger sets with the individual plant approach appears feasible and more informative compared to the bulk analysis we conducted