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Sawhorse-type diruthenium tetracarbonyl complexes containing porphyrin-derived ligands as highly selective photosensitizers for female reproductive cancer cells

Schmitt, Frédéric ; Auzias, Mathieu ; Štěpnička, Petr ; Sei, Yoshihisa ; Yamaguchi, Kentaro ; Süss-Fink, Georg ; Therrien, Bruno ; Juillerat-Jeanneret, Lucienne

In: JBIC Journal of Biological Inorganic Chemistry, 2009, vol. 14, no. 5, p. 693-701

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    Titre
    • Sawhorse-type diruthenium tetracarbonyl complexes containing porphyrin-derived ligands as highly selective photosensitizers for female reproductive cancer cells
    Auteur
    • Schmitt, Frédéric. Institut Universitaire de Pathologie, CHUV, Bugnon 25, 1011, Lausanne, Switzerland
    • Auzias, Mathieu. Institut de Chimie, Université de Neuchâtel, Case postale 158, 2009, Neuchâtel, Switzerland
    • Štěpnička, Petr. Department of Inorganic Chemistry, Faculty of Science, Charles University, Hlavova 2030, 12840, Prague 2, Czech Republic
    • Sei, Yoshihisa. Laboratory of Analytical Chemistry, Tokushima Bunri University, Shido, 769-2193, Sanuki, Kagawa, Japan
    • Yamaguchi, Kentaro. Laboratory of Analytical Chemistry, Tokushima Bunri University, Shido, 769-2193, Sanuki, Kagawa, Japan
    • Süss-Fink, Georg. Institut de Chimie, Université de Neuchâtel, Case postale 158, 2009, Neuchâtel, Switzerland
    • Therrien, Bruno. Institut de Chimie, Université de Neuchâtel, Case postale 158, 2009, Neuchâtel, Switzerland
    • Juillerat-Jeanneret, Lucienne. Institut Universitaire de Pathologie, CHUV, Bugnon 25, 1011, Lausanne, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • JBIC Journal of Biological Inorganic Chemistry, 2009, vol. 14, no. 5, p. 693-701. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:317633
    Summary
    Diruthenium tetracarbonyl complexes of the type [Ru2(CO)4(μ2-η2-O2CR)2L2] containing a Ru-Ru backbone with four equatorial carbonyl ligands, two carboxylato bridges, and two axial two-electron ligands in a sawhorse-like geometry have been synthesized with porphyrin-derived substituents in the axial ligands [1: R is CH3, L is 5-(4-pyridyl)-10,15,20-triphenyl-21,23H-porphyrin], in the bridging carboxylato ligands [2: RCO2H is 5-(4-carboxyphenyl)-10,15,20-triphenyl-21,23H-porphyrin, L is PPh3; 3: RCO2H is 5-(4-carboxyphenyl)-10,15,20-triphenyl-21,23H-porphyrin, L is 1,3,5-triaza-7-phosphatricyclo[3.3.1.1]decane], or in both positions [4: RCO2H is 5-(4-carboxyphenyl)-10,15,20-triphenyl-21,23H-porphyrin, L is 5-(4-pyridyl)-10,15,20-triphenyl-21,23H-porphyrin]. Compounds 1-3 were assessed on different types of human cancer cells and normal cells. Their uptake by cells was quantified by fluorescence and checked by fluorescence microscopy. These compounds were taken up by human HeLa cervix and A2780 and Ovcar ovarian carcinoma cells but not by normal cells and other cancer cell lines (A549 pulmonary, Me300 melanoma, PC3 and LnCap prostate, KB head and neck, MDAMB231 and MCF7 breast, or HT29 colon cancer cells). The compounds demonstrated no cytotoxicity in the absence of laser irradiation but exhibited good phototoxicities in HeLa and A2780 cells when exposed to laser light at 652nm, displaying an LD50 between 1.5 and 6.5J/cm2 in these two cell lines and more than 15J/cm2 for the others. Thus, these types of porphyric compound present specificity for cancer cell lines of the female reproductive system and not for normal cells; thus being promising new organometallic photosensitizers