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Sequence analysis of a DNA fragment from Sinorhizobium fredii USDA257 which extends the nitrogen fixation host range of Rhizobium species NGR234 to soybean, Glycine max (L.) Merr cultivar Peking

Boboye, B. ; Nyakatura, G. ; Rosenthal, A. ; Perret, X. ; Broughton, W. ; Boller, Th

In: Symbiosis, 2009, vol. 48, no. 1-3, p. 110-119

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    Title
    • Sequence analysis of a DNA fragment from Sinorhizobium fredii USDA257 which extends the nitrogen fixation host range of Rhizobium species NGR234 to soybean, Glycine max (L.) Merr cultivar Peking
    Author
    • Boboye, B.. Botanisches Institut der Universität Basel, Hebelstrasse 1, 4056, Basel, Switzerland
    • Nyakatura, G.. Abteilung Genomanalyse, Institut für Molekulare Biotechnologie, 07745, Jena, Germany
    • Rosenthal, A.. Abteilung Genomanalyse, Institut für Molekulare Biotechnologie, 07745, Jena, Germany
    • Perret, X.. Laboratoire de Biologie Moléculaire des Plantes Supérieures, Université de Genève, 1 Ch. de l'Impératrice, 1292, Chambésy/Genève, Switzerland
    • Broughton, W.. Laboratoire de Biologie Moléculaire des Plantes Supérieures, Université de Genève, 1 Ch. de l'Impératrice, 1292, Chambésy/Genève, Switzerland
    • Boller, Th. Botanisches Institut der Universität Basel, Hebelstrasse 1, 4056, Basel, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Symbiosis, 2009, vol. 48, no. 1-3, p. 110-119. Springer Netherlands
    Other Version
    OAI-PMH Identifier
    • oai:doc.rero.ch:316415
    Summary
    A fragment of DNA (pBTBX) from the genome ofSinorhizobium fredii USDA257 was sequenced by shotgun strategy to identify the potential genes which enabled theRhizobium species NGR234 to fix nitrogen on soybean,Glycine max (L.) Merr cv. Peking. The total length of the cosmid is 32,824 base pairs with a GC content of 61%. A 29 open reading frames (ORF) were identified representing 71.8% (23,574 bp) of the cosmid. Out of these ORF, 96.5% (22,749 bp) were identical and similar to reported and hypothetical genes and proteins. The remaining 3.5% (825 bp) had no apparent similarity to any genes in the data base. Gene and gene products found on the DNA fragment include those involved in the synthesis of FeMo component of nitrogenase, regulation of nitrogen fixation, transport of amino acids and sugars, chemotaxis and transcriptional regulation