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Segment-specific expression of sodium-phosphate cotransporters NaPi-IIa and -IIc and interacting proteins in mouse renal proximal tubules

Madjdpour*, C. ; Bacic*, D. ; Kaissling, B. ; Murer, H. ; Biber, J.

In: Pflügers Archiv, 2004, vol. 448, no. 4, p. 402-410

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    Titre
    • Segment-specific expression of sodium-phosphate cotransporters NaPi-IIa and -IIc and interacting proteins in mouse renal proximal tubules
    Auteur
    • Madjdpour*, C.. Institute of Anatomy, University of Zurich, 8057, Zurich, Switzerland
    • Bacic*, D.. Institute of Anatomy, University of Zurich, 8057, Zurich, Switzerland
    • Kaissling, B.. Institute of Anatomy, University of Zurich, 8057, Zurich, Switzerland
    • Murer, H.. Institute of Physiology, University of Zürich, Winterthurerstrasse 190, 8057, Zurich, Switzerland
    • Biber, J.. Institute of Physiology, University of Zürich, Winterthurerstrasse 190, 8057, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Pflügers Archiv, 2004, vol. 448, no. 4, p. 402-410. Springer-Verlag; http://www.springer.de
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:316299
    Summary
    Sodium-dependent phosphate cotransport in renal proximal tubules (PTs) is heterogeneous with respect to proximal tubular segmentation (S1 vs. S3) and nephron generation (superficial vs. juxtamedullary). In the present study, S1 and S3 segments of superficial and juxtamedullary nephrons were laser-microdissected and mRNA and protein expression of the Na/Pi-cotransporters NaPi-IIa and NaPi-IIc and the PDZ proteins NHERF-1 and PDZK1 determined. Expression of NaPi-IIa mRNA decreased axially in juxtamedullary nephrons. There was no effect of dietary Pi content on NaPi-lla mRNA expression in any proximal tubular segment. The abundance of the NaPi-IIa cotransporter in the brush-border membrane showed inter- and intranephron heterogeneity and increased in response to a low-Pi diet (5days), suggesting that up-regulation of NaPi-lla occurs via post-transcriptional mechanisms. In contrast, NaPi-IIc mRNA and protein was up-regulated by the low-Pi diet in all nephron generations analysed. NHERF-1 and PDZK1, at both mRNA and protein levels, were distributed evenly along the PTs and did not change after a low-Pi diet