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Molecular imaging by micro-CT: specific E-selectin imaging

Wyss, Caroline ; Schaefer, Stephan ; Juillerat-Jeanneret, Lucienne ; Lagopoulos, Lucienne ; Lehr, Hans-Anton ; Becker, Christoph ; Montet, Xavier

In: European Radiology, 2009, vol. 19, no. 10, p. 2487-2494

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    Titre
    • Molecular imaging by micro-CT: specific E-selectin imaging
    Auteur
    • Wyss, Caroline. University Institute of Pathology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    • Schaefer, Stephan. University Institute of Pathology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    • Juillerat-Jeanneret, Lucienne. University Institute of Pathology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    • Lagopoulos, Lucienne. University Institute of Pathology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    • Lehr, Hans-Anton. University Institute of Pathology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    • Becker, Christoph. Department of Radiology, University of Geneva, Micheli-du-Crest 24, 1211, Geneva, Switzerland
    • Montet, Xavier. Department of Radiology, University of Geneva, Micheli-du-Crest 24, 1211, Geneva, Switzerland
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:315072
    Summary
    The primary goal of this study was to design a fluorescent E-selectin-targeted iodine-containing liposome for specific E-selectin imaging with the use of micro-CT. The secondary goal was to correlate the results of micro-CT imaging with other imaging techniques with cellular resolution, i.e., confocal and intravital microscopy. E-selectin-targeted liposomes were tested on endothelial cells in culture and in vivo in HT-29 tumor-bearing mice (n = 12). The liposomes contained iodine (as micro-CT contrast medium) and fluorophore (as optical contrast medium) for confocal and intravital microscopy. Optical imaging methods were used to confirm at the cellular level, the observations made with micro-CT. An ischemia-reperfusion model was used to trigger neovessel formation for intravital imaging. The E-selectin-targeted liposomes were avidly taken up by activated endothelial cells, whereas nontargeted liposomes were not. Direct binding of the E-selectin-targeted liposomes was proved by intravital microscopy, where bright spots clearly appeared on the activated vessels. Micro-CT imaging also demonstrated accumulation of the targeted lipsomes into subcutaneous tumor by an increase of 32 ± 8HU. Hence, internalization by activated endothelial cells was rapid and mediated by E-selectin. We conclude that micro-CT associated with specific molecular contrast agent is able to detect specific molecular markers on activated vessel walls in vivo