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DNA degradation in avian faecal samples and feasibility of non-invasive genetic studies of threatened capercaillie populations

Regnaut, Sébastien ; Lucas, Françoise ; Fumagalli, Luca

In: Conservation Genetics, 2006, vol. 7, no. 3, p. 449-453

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    Titre
    • DNA degradation in avian faecal samples and feasibility of non-invasive genetic studies of threatened capercaillie populations
    Auteur
    • Regnaut, Sébastien. Department of Ecology and Evolution, Laboratory for Conservation Biology, University of Lausanne, CH-1015, Lausanne, Switzerland
    • Lucas, Françoise. Department of Ecology and Evolution, Laboratory for Conservation Biology, University of Lausanne, CH-1015, Lausanne, Switzerland
    • Fumagalli, Luca. Department of Ecology and Evolution, Laboratory for Conservation Biology, University of Lausanne, CH-1015, Lausanne, Switzerland
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:314698
    Summary
    We evaluated the feasibility of using faeces as a non-invasively collected DNA source for the genetic study of an endangered bird population (capercaillie; Tetrao urogallus). We used a multitube approach, and for our panel of 11 microsatellites genotyping reliability was estimated at 98% with five repetitions. Experiments showed that free DNases in faecal material were the major cause of DNA degradation. Our results demonstrate that using avian faeces as a source of DNA, reliable microsatellite genotyping can be obtained with a reasonable number of PCR replicates