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Comparative copro-diagnosis of Echinococcus multilocularis in experimentally infected foxes

Al-Sabi', M. ; Kapel, C. ; Deplazes, P. ; Mathis, A.

In: Parasitology Research, 2007, vol. 101, no. 3, p. 731-736

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    Titre
    • Comparative copro-diagnosis of Echinococcus multilocularis in experimentally infected foxes
    Auteur
    • Al-Sabi', M.. Danish Centre for Experimental Parasitology, Faculty of Life Science, Copenhagen University, Dyrlaegevej 100, 1870, Frederiksberg, Denmark
    • Kapel, C.. Institute of Zoology, Department of Ecology, Faculty of Life Science, Copenhagen University, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark
    • Deplazes, P.. Institute of Parasitology, University of Zurich, Winterthurerstrasse 266A, CH-8057, Zurich, Switzerland
    • Mathis, A.. Institute of Parasitology, University of Zurich, Winterthurerstrasse 266A, CH-8057, Zurich, Switzerland
    Type de document
    • Postprint
    Classification
    • Biologie, Sciences de la vie
    Identifiant OAI-PMH
    • oai:doc.rero.ch:313783
    Summary
    Faecal samples from 15 foxes experimentally infected with Echinococcus multilocularis were examined until 90days post-infection (dpi) by microscopical identification of eggs isolated by flotation/sieving, by coproantigen-enzyme-linked immunosorbent assay (cELISA), by polymerase chain reaction (PCR) on DNA, respectively, isolated directly from the faecal samples (copro-DNA PCR) and from the eggs obtained by the flotation/sieving procedure (egg-DNA PCR). Based on egg counts, three periods of the infection were defined: pre-patent (2-29dpi), high patent (30-70dpi) and low patent periods (71-90dpi). Whereas all methods were highly sensitive with samples from the high patent period, cELISA was the most sensitive to detect pre-patent infections (63%). Samples from the low patent infections were positive in 77% by microscopy and in 80% by egg-DNA PCR, being significantly more sensitive than cELISA and copro-DNA PCR. The isolation of eggs from the faecal material proved to be more sensitive by the flotation/sieving procedure as compared to the classical concentration McMaster technique