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Characterization of hypovirulent isolates of the chestnut blight fungus, Cryphonectria parasitica from the Marmara and Black Sea regions of Turkey

Akıllı, Seçil ; Ulubaş Serçe, Çiğdem ; Katırcıoğlu, Yakup ; Maden, Salih ; Rigling, Daniel

In: European Journal of Plant Pathology, 2013, vol. 135, no. 2, p. 323-334

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    Titre
    • Characterization of hypovirulent isolates of the chestnut blight fungus, Cryphonectria parasitica from the Marmara and Black Sea regions of Turkey
    Auteur
    • Akıllı, Seçil. Faculty of Science, Department of Biology, Çankırı Karatekin University, Çankırı, Turkey
    • Ulubaş Serçe, Çiğdem. Agricultural Faculty, Department of Plant Protection, Mustafa Kemal University, 31034, Hatay, Turkey
    • Katırcıoğlu, Yakup. Agricultural Faculty, Department of Plant Protection, Ankara University, 06110, Ankara, Dışkapı, Turkey
    • Maden, Salih. Agricultural Faculty, Department of Plant Protection, Ankara University, 06110, Ankara, Dışkapı, Turkey
    • Rigling, Daniel. Swiss Federal Institute for Forest, Snow and Landscape Research (WSL), Zürcherstrasse 111, 8903, Birmensdorf, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • European Journal of Plant Pathology, 2013, vol. 135, no. 2, p. 323-334. Springer Netherlands
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:312663
    Summary
    Chestnut blight caused by the introduced fungus Cryphonectria parasitica has been responsible for the decline of Castanea sativa in Turkey since the 1960s. In this study, 72 C. parasitica isolates were recovered from the Marmara and Black Sea regions of Turkey showing white or cream-coloured culture morphology and were subjected to various tests to determine if they were infected by Cryphonectria hypovirus 1 (CHV-1). The vast majority of the isolates (69 out of 72) were vc type EU-1. Both mating types were found among a subsample of the isolates. The hypovirus was detected in 55 isolates by dsRNA extraction and/or virus specific RT-PCR on total RNA extracts. All but one isolates showed no or only weak phenol oxidase activity on agar medium containing tannic acid, typical of CHV-1 infected isolates. Through sequencing of a specific region of the hypovirus genome, we found that 24 hypovirus isolates belonged to the CHV-1 subtype I and six to the CHV-1 subtype F2. The distribution of the two CHV-1 subtypes in Turkey showed a clear geographic pattern. CHV-1 subtype I was only detected in the Marmara and western Black Sea region, whereas subtype F2 was restricted to the eastern part of the Black Sea region. The effectiveness of 23 hypovirulent isolates was tested against a virulent isolate on 2-3years old chestnut sprouts. Ten hypovirulent isolates, all infected by CHV-1 subtype I, prevented canker development by more than 80% suggesting that they might be suitable for biological control of chestnut blight in Turkey