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Construction and characterization of a BAC-library for a key pollinator, the bumblebee Bombus terrestris L

Wilfert, L. ; Torres, M. ; Reber-Funk, C. ; Schmid-Hempel, R. ; Tomkins, J. ; Gadau, J. ; Schmid-Hempel, P.

In: Insectes Sociaux, 2009, vol. 56, no. 1, p. 44-48

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    Titre
    • Construction and characterization of a BAC-library for a key pollinator, the bumblebee Bombus terrestris L
    Auteur
    • Wilfert, L.. Institute of Integrative Biology (IBZ), ETH Zurich, CH-8092, Zurich, Switzerland
    • Torres, M.. Clemson University Genomics Institute, Clemson University, 29634, Clemson, SC, USA
    • Reber-Funk, C.. Institute of Integrative Biology (IBZ), ETH Zurich, CH-8092, Zurich, Switzerland
    • Schmid-Hempel, R.. Institute of Integrative Biology (IBZ), ETH Zurich, CH-8092, Zurich, Switzerland
    • Tomkins, J.. Clemson Environmental Genomics Lab, Clemson University, 29634, Clemson, SC, USA
    • Gadau, J.. School of Life Sciences, Arizona State University, 85287-4501, Tempe, AZ, USA
    • Schmid-Hempel, P.. Institute of Integrative Biology (IBZ), ETH Zurich, CH-8092, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Insectes Sociaux, 2009, vol. 56, no. 1, p. 44-48. Birkhäuser-Verlag; www.birkhäuser.ch
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:312217
    Summary
    Abstract.: The primitively social bumblebee Bombus terrestris is an ecological model species as well as an important agricultural pollinator. As part of the ongoing development of genomic resources for this model organism, we have constructed a publicly available bacterial artificial chromosome (BAC) library from males of a field-derived colony. We have shown that this library has a high coverage, which allows any particular sequence to be retrieved from at least one clone with a probability of 99.7%. We have further demonstrated the library's usefulness by successfully screening it with probes derived both from previously described B. terrestris genes and candidate genes from another bumblebee species and the honeybee. This library will facilitate genomic studies in B. terrestris and will allow for novel comparative studies in the social Hymenoptera