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MRSA screening by the Xpert MRSA PCR assay: pooling samples of the nose, throat, and groin increases the sensitivity of detection without increasing the laboratory costs

Blanc, D. ; Nahimana, I. ; Zanetti, G. ; Greub, G.

In: European Journal of Clinical Microbiology & Infectious Diseases, 2013, vol. 32, no. 4, p. 565-568

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    Titre
    • MRSA screening by the Xpert MRSA PCR assay: pooling samples of the nose, throat, and groin increases the sensitivity of detection without increasing the laboratory costs
    Auteur
    • Blanc, D.. Service of Hospital Preventive Medicine, Lausanne University Hospital, Rue du Bugnon 46, 1011, Lausanne, Switzerland
    • Nahimana, I.. Service of Hospital Preventive Medicine, Lausanne University Hospital, Rue du Bugnon 46, 1011, Lausanne, Switzerland
    • Zanetti, G.. Service of Hospital Preventive Medicine, Lausanne University Hospital, Rue du Bugnon 46, 1011, Lausanne, Switzerland
    • Greub, G.. Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • European Journal of Clinical Microbiology & Infectious Diseases, 2013, vol. 32, no. 4, p. 565-568. Springer-Verlag
    Autre version électronique
    Classification
    • Santé
    Identifiant OAI-PMH
    • oai:doc.rero.ch:311987
    Summary
    The performance of the Xpert MRSA polymerase chain reaction (PCR) assay on pooled nose, groin, and throat swabs (three nylon flocked eSwabs into one tube) was compared to culture by analyzing 5,546 samples. The sensitivity [0.78, 95% confidence interval (CI) 0.73-0.82] and specificity (0.99, 95% CI 0.98-0.99) were similar to the results from published studies on separated nose or other specimens. Thus, the performance of the Xpert MRSA assay was not affected by pooling the three specimens into one assay, allowing a higher detection rate without increasing laboratory costs, as compared to nose samples alone