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Micropositioning and microscopic observation of individual picoliter-sized containers within SU-8 microchannels

Jenke, Martin ; Schreiter, Christoph ; Kim, Gyu ; Vogel, Horst ; Brugger, Jürgen

In: Microfluidics and Nanofluidics, 2007, vol. 3, no. 2, p. 189-194

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    Titre
    • Micropositioning and microscopic observation of individual picoliter-sized containers within SU-8 microchannels
    Auteur
    • Jenke, Martin. Microsystems Laboratory (LMIS), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland
    • Schreiter, Christoph. Laboratory of Physical Chemistry of Polymers and Membranes (LCPPM), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland
    • Kim, Gyu. Microsystems Laboratory (LMIS), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland
    • Vogel, Horst. Laboratory of Physical Chemistry of Polymers and Membranes (LCPPM), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland
    • Brugger, Jürgen. Microsystems Laboratory (LMIS), Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Microfluidics and Nanofluidics, 2007, vol. 3, no. 2, p. 189-194. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:310518
    Summary
    We describe the fabrication and application of a bioanalytical chip, made of SU-8 photoresist, comprising integrated, high aspect-ratio microfluidic channels, suitable to manipulate and investigate vesicles, cell fragments and biological cells. A central micrometer-sized aperture allows electrical particle counting and planar membrane experiments, microfluids allow (sub)micrometer-sized objects to be transported and addressed with different chemicals. Here we show how lipid vesicles are positioned with micrometer precision within the micro-channels by means of pressure and electrophoretic movement. Our approach is suited for controlling and investigating (bio)chemical synthesis and cellular signalling processes in ultrasmall individual vesicles by electro-optical techniques