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Electron capture and transfer dissociation: Peptide structure analysis at different ion internal energy levels

Hamidane, Hisham ; Chiappe, Diego ; Hartmer, Ralf ; Vorobyev, Aleksey ; Moniatte, Marc ; Tsybin, Yury

In: Journal of the American Society for Mass Spectrometry, 2009, vol. 20, no. 4, p. 567-575

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    Titre
    • Electron capture and transfer dissociation: Peptide structure analysis at different ion internal energy levels
    Auteur
    • Hamidane, Hisham. Ecole Polytechnique Fédérale de Lausanne, Biomolecular Mass Spectrometry Laboratory, BCH 4307, 1015, Lausanne, Switzerland
    • Chiappe, Diego. Proteomics Core Facility, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
    • Hartmer, Ralf. Bruker Daltonics GmbH, Bremen, Germany
    • Vorobyev, Aleksey. Ecole Polytechnique Fédérale de Lausanne, Biomolecular Mass Spectrometry Laboratory, BCH 4307, 1015, Lausanne, Switzerland
    • Moniatte, Marc. Proteomics Core Facility, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
    • Tsybin, Yury. Ecole Polytechnique Fédérale de Lausanne, Biomolecular Mass Spectrometry Laboratory, BCH 4307, 1015, Lausanne, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Journal of the American Society for Mass Spectrometry, 2009, vol. 20, no. 4, p. 567-575. Springer-Verlag
    Autre version électronique
    Classification
    • Chimie
    Identifiant OAI-PMH
    • oai:doc.rero.ch:309630
    Summary
    We decoupled electron-transfer dissociation (ETD) and collision-induced dissociation of charge-reduced species (CRCID) events to probe the lifetimes of intermediate radical species in ETD-based ion trap tandem mass spectrometry of peptides. Short-lived intermediates formed upon electron transfer require less energy for product ion formation and appear in regular ETD mass spectra, whereas long-lived intermediates require additional vibrational energy and yield product ions as a function of CRCID amplitude. The observed dependencies complement the results obtained by double-resonance electron-capture dissociation (ECD) Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) and ECD in a cryogenic ICR trap. Compared with ECD FT-ICR MS, ion trap MS offers lower precursor ion internal energy conditions, leading to more abundant charge-reduced radical intermediates and larger variation of product ion abundance as a function of vibrational post-activation amplitude. In many cases decoupled CRCID after ETD exhibits abundant radical c-type and even-electron z-type ions, in striking contrast to predominantly even-electron c-type and radical z-type ions in ECD FT-ICR MS and especially activated ion-ECD, thus providing a new insight into the fundamentals of ECD/ETD