Fulltext

Integration of the blaNDM-1 carbapenemase gene into Proteus genomic island 1 (PGI1-PmPEL) in a Proteus mirabilis clinical isolate

Girlich, Delphine ; Dortet, Laurent ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2015, vol. 70, no. 1, p. 98-102

Ajouter à la liste personnelle
    Titre
    • Integration of the blaNDM-1 carbapenemase gene into Proteus genomic island 1 (PGI1-PmPEL) in a Proteus mirabilis clinical isolate
    Auteur
    • Girlich, Delphine. INSERM U914 ‘Emerging Resistance to Antibiotics', Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
    • Dortet, Laurent. INSERM U914 ‘Emerging Resistance to Antibiotics', Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
    • Poirel, Laurent. INSERM U914 ‘Emerging Resistance to Antibiotics', Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
    • Nordmann, Patrice. INSERM U914 ‘Emerging Resistance to Antibiotics', Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Journal of Antimicrobial Chemotherapy, 2015, vol. 70, no. 1, p. 98-102. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:304511
    Summary
    Objectives To decipher the mechanisms and their associated genetic determinants responsible for β-lactam resistance in a Proteus mirabilis clinical isolate. Methods The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking and DNA sequencing. Results Genes encoding the carbapenemase NDM-1 and the ESBL VEB-6 were located in a 38.5 kb MDR structure, which itself was inserted into a new variant of the Proteus genomic island 1 (PGI1). This new PGI1-PmPEL variant of 64.4 kb was chromosomally located, as an external circular form in the P. mirabilis isolate, suggesting potential mobility. Conclusions This is the first known description of the blaNDM-1 gene in a genomic island structure, which might further enhance the spread of the blaNDM-1 carbapenemase gene among enteric pathogens