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Green fluorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum

Rekangalt, David ; Verner, Marie-Christine ; Kües, Ursula ; Walser, Piers James ; Marmeisse, Roland ; Debaud, Jean-Claude ; Fraissinet-Tachet, Laurence

In: FEMS Microbiology Letters, 2007, vol. 268, no. 1, p. 67-72

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    Titre
    • Green fluorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum
    Auteur
    • Rekangalt, David. Université de Lyon, Université Lyon 1, Ecologie Microbienne (UMR CNRS 5557, USC INRA 1193), Villeurbanne, France
    • Verner, Marie-Christine. Université de Lyon, Université Lyon 1, Ecologie Microbienne (UMR CNRS 5557, USC INRA 1193), Villeurbanne, France
    • Kües, Ursula. Georg-August-University Göttingen, Institute for Forest Botany, Molecular Wood Biotechnology, Göttingen, Germany
    • Walser, Piers James. Institute of Microbiology, ETH Zürich, Zürich, Switzerland
    • Marmeisse, Roland. Université de Lyon, Université Lyon 1, Ecologie Microbienne (UMR CNRS 5557, USC INRA 1193), Villeurbanne, France
    • Debaud, Jean-Claude. Université de Lyon, Université Lyon 1, Ecologie Microbienne (UMR CNRS 5557, USC INRA 1193), Villeurbanne, France
    • Fraissinet-Tachet, Laurence. Université de Lyon, Université Lyon 1, Ecologie Microbienne (UMR CNRS 5557, USC INRA 1193), Villeurbanne, France
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:304401
    Summary
    The symbiotic basidiomycete Hebeloma cylindrosporum is a model fungal species used to study ectomycorrhizal symbiosis at the molecular level. In order to have a vital marker, we developed a green fluorescent protein (GFP) reporter system efficiently expressed in H. cylindrosporum using the sgfp coding region bordered by two introns fused to the saprophytic basidiomycete Coprinopsis cinerea cgl1 promoter. Expression of this reporter system was tested under different environmental conditions in two transformants, and glucose was shown to repress gfp expression. Such a reporter system will be used in plant-fungus interaction to evaluate sugar supply by the plant to the compatible mycorrhizal symbiont and to compare the expression of various genes of interest in the free-living mycelia, in the symbiotic (mycorrhizas) and the reproductive (fruit bodies) structures formed by H. cylindrosporum