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Daptomycin resistance mechanisms in clinically derived Staphylococcus aureus strains assessed by a combined transcriptomics and proteomics approach

Fischer, Adrien ; Yang, Soo-Jin ; Bayer, Arnold S. ; Vaezzadeh, Ali R. ; Herzig, Sébastien ; Stenz, Ludwig ; Girard, Myriam ; Sakoulas, George ; Scherl, Alexander ; Yeaman, Michael R. ; Proctor, Richard A. ; Schrenzel, Jacques ; François, Patrice

In: Journal of Antimicrobial Chemotherapy, 2011, vol. 66, no. 8, p. 1696-1711

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    Titre
    • Daptomycin resistance mechanisms in clinically derived Staphylococcus aureus strains assessed by a combined transcriptomics and proteomics approach
    Auteur
    • Fischer, Adrien. Genomic Research Laboratory, University Hospitals of Geneva and University of Geneva, Geneva, Switzerland
    • Yang, Soo-Jin. LA Biomedical Research Institute, Torrance, CA, USA
    • Bayer, Arnold S.. LA Biomedical Research Institute, Torrance, CA, USA
    • Vaezzadeh, Ali R.. Harvard Medical School, Boston, MA, USA
    • Herzig, Sébastien. Genomic Research Laboratory, University Hospitals of Geneva and University of Geneva, Geneva, Switzerland
    • Stenz, Ludwig. Genomic Research Laboratory, University Hospitals of Geneva and University of Geneva, Geneva, Switzerland
    • Girard, Myriam. Genomic Research Laboratory, University Hospitals of Geneva and University of Geneva, Geneva, Switzerland
    • Sakoulas, George. Sharp Medical Center, San Diego, CA, USA
    • Scherl, Alexander. BPRG, University Hospitals of Geneva, Geneva, Switzerland
    • Yeaman, Michael R.. LA Biomedical Research Institute, Torrance, CA, USA
    • Proctor, Richard A.. University of Wisconsin School of Medicine, Madison, WI, USA
    • Schrenzel, Jacques. Genomic Research Laboratory, University Hospitals of Geneva and University of Geneva, Geneva, Switzerland
    • François, Patrice. Genomic Research Laboratory, University Hospitals of Geneva and University of Geneva, Geneva, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Journal of Antimicrobial Chemotherapy, 2011, vol. 66, no. 8, p. 1696-1711. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:301593
    Summary
    Objectives The development of daptomycin resistance in Staphylococcus aureus is associated with clinical treatment failures. The mechanism(s) of such resistance have not been clearly defined. Methods We studied an isogenic daptomycin-susceptible (DAPS) and daptomycin-resistant (DAPR) S. aureus strain pair (616; 701) from a patient with relapsing endocarditis during daptomycin treatment, using comparative transcriptomic and proteomic techniques. Results Minor differences in the genome content were found between strains by DNA hybridization. Transcriptomic analyses identified a number of genes differentially expressed in important functional categories: cell division; metabolism of bacterial envelopes; and global regulation. Of note, the DAPR isolate exhibited reduced expression of the major cell wall autolysis gene coincident with the up-regulation of genes involved in cell wall teichoic acid production. Using quantitative (q)RT-PCR on the gene cadre putatively involved in cationic peptide resistance, we formulated a putative regulatory network compatible with microarray data sets, mainly implicating bacterial envelopes. Of interest, qRT-PCR of this same gene cadre from two distinct isogenic DAPS/DAPR clinical strain pairs revealed evidence of other strain-dependent networks operative in the DAPR phenotype. Comparative proteomics of 616 versus 701 revealed a differential abundance of proteins in various functional categories, including cell wall-associated targets and biofilm formation proteins. Phenotypically, strains 616 and 701 showed major differences in their ability to develop bacterial biofilms in the presence of the antibacterial lipid, oleic acid. Conclusions Compatible with previous in vitro observations, in vivo-acquired DAPR in S. aureus is a complex, multistep phenomenon involving: (i) strain-dependent phenotypes; (ii) transcriptome adaptation; and (iii) modification of the lipid and protein contents of cellular envelopes