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Overexpression of the p73 gene is a novel finding in high-risk B-cell chronic lymphocytic leukemia

Novak, U. ; Grob, T. J. ; Baskaynak, G. ; Peters, U. R. ; Aebi, S. ; Zwahlen, D. ; Tschan, M. P. ; Kreuzer, K.-A ; Leibundgut, E. Oppliger ; Cajot, J.-F ; Tobler, A. ; Fey, M. F.

In: Annals of Oncology, 2001, vol. 12, no. 7, p. 981-986

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    Titre
    • Overexpression of the p73 gene is a novel finding in high-risk B-cell chronic lymphocytic leukemia
    Auteur
    • Novak, U.. University and Inselspital, Department of Clinical Research, Medical Oncology/Haematology Berne. Switzerland
    • Grob, T. J.. University and Inselspital, Department of Clinical Research, Medical Oncology/Haematology Berne. Switzerland
    • Baskaynak, G.. Charite/Virchow-Clinic, Humboldt-University, Department of Haematology/Oncology Berlin, Germany
    • Peters, U. R.. Charite/Virchow-Clinic, Humboldt-University, Department of Haematology/Oncology Berlin, Germany
    • Aebi, S.. University and Inselspital, Department of Clinical Research, Medical Oncology/Haematology Berne. Switzerland
    • Zwahlen, D.. University and Inselspital, Department of Clinical Research, Medical Oncology/Haematology Berne. Switzerland
    • Tschan, M. P.. University and Inselspital, Department of Clinical Research, Medical Oncology/Haematology Berne. Switzerland
    • Kreuzer, K.-A. Charite/Virchow-Clinic, Humboldt-University, Department of Haematology/Oncology Berlin, Germany
    • Leibundgut, E. Oppliger. University and Inselspital, Department of Clinical Research, Medical Oncology/Haematology Berne. Switzerland
    • Cajot, J.-F. University and Inselspital, Department of Clinical Research, Medical Oncology/Haematology Berne. Switzerland
    • Tobler, A.. University and Inselspital, Department of Clinical Research, Medical Oncology/Haematology Berne. Switzerland
    • Fey, M. F.. University and Inselspital, Department of Clinical Research, Medical Oncology/Haematology Berne. Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Annals of Oncology, 2001, vol. 12, no. 7, p. 981-986. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:300715
    Summary
    The p73 protein shares structural and functional similarities with the tumour-suppressor p53, but its role in neoplastic transformation is unknown. Alternative splicing leads to the expression of at least nine p73 C-terminal mRNA splice variants (α β γ δ ε ξ η ηl θ). In this survey, we analyse the expression of p73 by real-time quantitative RT-PCR, its known C-terminal variants with an RT-PCR-Southern tech nique and by Western blot in samples of 51 patients with B-CLL, normal B lymphocytes from eight individuals, and five haematopoetic cell lines. p73α protein expression positively correlated with higher risk B-CLL stages (P=0.046). Total p73 mRNA expression was higher (P= 0.01) and p73α protein more frequently detected (P=0.008) in B-CLL compared with normal CD19+—B-lymphocytes. p73 C-terminal mRNA variants were expressed both in B-CLL and in normal B-lymphocytes, but their expression was biased since the γ (P=0.041), the θ (P ≪ 0.001), and the η variant (P=0.033) prevailed in normal B-lymphocytes. In summary, we conclude that the accumulation of p73, the expression pattern of particular p73 variants and its link to progression may play a distinct role in the molecular pathology B-CLL