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The Escherichia coli glycophage display system

Dürr, Clemens ; Nothaft, Harald ; Lizak, Christian ; Glockshuber, Rudi ; Aebi, Markus

In: Glycobiology, 2010, vol. 20, no. 11, p. 1366-1372

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    Titre
    • The Escherichia coli glycophage display system
    Auteur
    • Dürr, Clemens. Institute of Microbiology, Department of Biology
    • Nothaft, Harald. Institute of Microbiology, Department of Biology
    • Lizak, Christian. Institute of Microbiology, Department of Biology
    • Glockshuber, Rudi. Institute of Molecular Biology and Biophysics, Department of Biology, Swiss Federal Institute of Technology, ETH Zurich, CH-8093 Zurich, Switzerland
    • Aebi, Markus. Institute of Microbiology, Department of Biology
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Glycobiology, 2010, vol. 20, no. 11, p. 1366-1372. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:299293
    Summary
    We describe a phage display technique that allows the production and selective enrichment of phages that display an N-glycoprotein (glycophages). We applied glycophage display to select functional glycosylation sequons from a pool of randomized acceptor sequences. Our system provides a genetic platform to study and engineer different steps in the pathway of bacterial N-linked protein glycosylation