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Copper acquisition by the SenC protein regulates aerobic respiration in Pseudomonas aeruginosa PAO1

Frangipani, Emanuela ; Haas, Dieter

In: FEMS Microbiology Letters, 2009, vol. 298, no. 2, p. 234-240

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    Titre
    • Copper acquisition by the SenC protein regulates aerobic respiration in Pseudomonas aeruginosa PAO1
    Auteur
    • Frangipani, Emanuela. Département de Microbiologie Fondamentale, Université de Lausanne, Lausanne, Switzerland
    • Haas, Dieter. Département de Microbiologie Fondamentale, Université de Lausanne, Lausanne, Switzerland
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:299157
    Summary
    Aerobic respiration of Pseudomonas aeruginosa involves four terminal oxidases belonging to the heme-copper family (that is, three cytochrome c oxidases and one quinol oxidase) plus one copper-independent, cyanide-insensitive quinol oxidase (CIO). The PA0114 gene encoding an SCO1/SenC-type protein, which is known to be important for copper delivery to cytochrome c in yeast, Rhodobacter spp. and Agrobacterium tumefaciens, was found to be important for copper acquisition and aerobic respiration in P. aeruginosa. A PA0114 (senC) mutant grew poorly in low-copper media and had low cytochrome cbb3-type oxidase activity, but expressed CIO at increased levels, by comparison with the wild-type PAO1. Addition of copper reversed these phenotypes, suggesting that periplasmic copper capture by the SenC protein helps P. aeruginosa to adapt to copper deprivation