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Atomic Force Microscopy Imaging of Living Cells

Berquand, Alexandre ; Roduit, Charles ; Kasas, Sandor ; Holloschi, Andreas ; Ponce, Leslie ; Hafner, Mathias

In: Microscopy Today, 2010, vol. 18, no. 6, p. 8-14

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    Titre
    • Atomic Force Microscopy Imaging of Living Cells
    Auteur
    • Berquand, Alexandre. Veeco Instruments GmbH, Dynamostrasse 19, 68165 Mannheim, Germany
    • Roduit, Charles. Institut de Physique des Systèmes Biologiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
    • Kasas, Sandor. Institut de Physique des Systèmes Biologiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
    • Holloschi, Andreas. University of Applied Sciences, Institute of Molecular and Cell Biology, Mannheim, Germany
    • Ponce, Leslie. University of Applied Sciences, Institute of Molecular and Cell Biology, Mannheim, Germany
    • Hafner, Mathias. University of Applied Sciences, Institute of Molecular and Cell Biology, Mannheim, Germany
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:298419
    Summary
    Over the last two decades, Atomic Force Microscopy (AFM) has emerged as the tool of choice to image living organisms in a near-physiological environment. Whereas fluorescence microscopy techniques allow labeling and tracking of components inside cells and the observation of dynamic processes, AFM is mainly a surface technique that can be operated on a wide range of substrates including biological samples. AFM enables extraction of topographical, mechanical and chemical information from these samples