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Seasonality in bacterial diversity in north-west Mediterranean coastal waters: assessment through clone libraries, fingerprinting and FISH

Alonso-Sáez, Laura ; Balagué, Vanessa ; Sà, Elisabet L. ; Sánchez, Olga ; González, José M. ; Pinhassi, Jarone ; Massana, Ramon ; Pernthaler, Jakob ; Pedrós-Alió, Carlos ; Gasol, Josep M.

In: FEMS Microbiology Ecology, 2007, vol. 60, no. 1, p. 98-112

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    Titre
    • Seasonality in bacterial diversity in north-west Mediterranean coastal waters: assessment through clone libraries, fingerprinting and FISH
    Auteur
    • Alonso-Sáez, Laura. Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar CSIC, Barcelona, Catalunya, Spain
    • Balagué, Vanessa. Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar CSIC, Barcelona, Catalunya, Spain
    • Sà, Elisabet L.. Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar CSIC, Barcelona, Catalunya, Spain
    • Sánchez, Olga. Departament de Genètica i Microbiologia. Universitat Autonoma de Barcelona, Bellaterra, Catalunya, Spain
    • González, José M.. Departamento de Microbiología, Universidad de La Laguna, La Laguna, Tenerife, Spain
    • Pinhassi, Jarone. Marine Microbiology, Department of Biology and Environmental Sciences, University of Kalmar, Kalmar, Sweden
    • Massana, Ramon. Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar CSIC, Barcelona, Catalunya, Spain
    • Pernthaler, Jakob. Limnological Station, Institute of Plant Biology, University of Zurich, Switzerland
    • Pedrós-Alió, Carlos. Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar CSIC, Barcelona, Catalunya, Spain
    • Gasol, Josep M.. Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar CSIC, Barcelona, Catalunya, Spain
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:298110
    Summary
    We combined denaturing gradient gel electrophoresis (DGGE), catalysed reporter deposition-FISH (CARD-FISH) and clone libraries to investigate the seasonality of the bacterial assemblage composition in north-west Mediterranean coastal waters. DGGE analysis indicated that bacterial diversity changed gradually throughout the year, although with a clear distinction of the summer period. Alphaproteobacteria were the dominant group on an annual basis [29% of the DAPI (4′,6-diamidino-2-phenylindole) counts by CARD-FISH, and 70% of the bacterial clones]. The SAR11 clade was most abundant during spring and summer (>20% of DAPI counts), while the Roseobacter clade was abundant primarily in winter and spring (up to 7% of DAPI counts). The phylum Bacteroidetes constituted the second most important group and was quantitatively uniform throughout the year (average 11% of the DAPI counts). Gammaproteobacteria showed a peak during summer (8% of DAPI counts), when most of them belonged to the NOR5 cluster. Clone libraries and CARD-FISH showed reasonable agreement in the quantitative proportions of Bacteroidetes and Gammaproteobacteria, but Alphaproteobacteria were overrepresented in clone libraries. Sequencing of the most predominant DGGE bands failed to detect the SAR11 group despite their high abundance. The combination of the three molecular approaches allowed a comprehensive assessment of seasonal changes in bacterial diversity