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Abnormal chromosomal arrangements in human oocytes

Macas, E. ; Floresheim, Y. ; Hotz, E. ; Imthurn, B. ; Keller, P.J. ; Walt, H.

In: Human Reproduction, 1990, vol. 5, no. 6, p. 703-707

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    Titre
    • Abnormal chromosomal arrangements in human oocytes
    Auteur
    • Macas, E.. Laboratory of Reproduction and Development, Department of Gynaecology and Obstetrics, University Hospital, CH-8091 Zurich, Switzerland
    • Floresheim, Y.. Laboratory of Reproduction and Development, Department of Gynaecology and Obstetrics, University Hospital, CH-8091 Zurich, Switzerland
    • Hotz, E.. Laboratory of Reproduction and Development, Department of Gynaecology and Obstetrics, University Hospital, CH-8091 Zurich, Switzerland
    • Imthurn, B.. Laboratory of Reproduction and Development, Department of Gynaecology and Obstetrics, University Hospital, CH-8091 Zurich, Switzerland
    • Keller, P.J.. Laboratory of Reproduction and Development, Department of Gynaecology and Obstetrics, University Hospital, CH-8091 Zurich, Switzerland
    • Walt, H.. Laboratory of Reproduction and Development, Department of Gynaecology and Obstetrics, University Hospital, CH-8091 Zurich, Switzerland
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:297992
    Summary
    Ninety-one human oocytes, lacking signs of fertilization 50 h after insemination in vitro, were investigated cytogenetically to assess the frequency and type of chromosomal abnormalities. Chromosome spreading permitted adequate karyotyping in 55 oocytes. Non-determined numerical aberrations occurred with the following frequencies: hypohaploidy, 10.9% (6/55), hyperhapJoidy, 14.5% (8/55) and hyperdiploidy, 3.6% (2/55). Total aneuploidy occurred with a frequency of 29.1% and was observed in oocytes from 30 patients. No correlation was found between specific chromosomal aberrations and type of infertility, stimulation treatment or gonadotrophin levels. On the other hand, the frequency of aneuploidy was significantly higher (P < 0.05) in patients >35 years of age. Two chromosomal complements (3.6%) had structural rearrangements; one oocyte had both structural and numerical chromosomal abnormalities and the other had differently condensed regions on the long arms of three chromosomes from group C. The overall frequency of chromosomal aberrations was 32.7%. Only two samples contained an additional set of polar body chromosomes. Thirteen oocytes presented sperm chromosomes in an arrested stage of premature chromosome condensation of the G1, phase and four oocytes showed asynchronous condensation of pronuclear chromosomes. Finally, it was concluded that the high proportion of chromosomal aberrations observed in human oocytes may contribute significantly to abnormal embryonic development in vitro