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Isolation and characterization of an inhibitory human monoclonal antibody specific to the urokinase-type plasminogen activator, uPA

Sgier, David ; Zuberbuehler, Kathrin ; Pfaffen, Stefanie ; Neri, Dario

In: Protein Engineering, Design & Selection, 2010, vol. 23, no. 4, p. 261-269

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    Titre
    • Isolation and characterization of an inhibitory human monoclonal antibody specific to the urokinase-type plasminogen activator, uPA
    Auteur
    • Sgier, David. ETH Zürich HCI G396, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Wolfgang-Pauli-Strasse 10 CH-8093 Zürich, Switzerland
    • Zuberbuehler, Kathrin. ETH Zürich HCI G396, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Wolfgang-Pauli-Strasse 10 CH-8093 Zürich, Switzerland
    • Pfaffen, Stefanie. ETH Zürich HCI G396, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Wolfgang-Pauli-Strasse 10 CH-8093 Zürich, Switzerland
    • Neri, Dario. E-mail: dario.neri@pharma.ethz.ch
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Protein Engineering, Design & Selection, 2010, vol. 23, no. 4, p. 261-269. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:296450
    Summary
    The serine protease urokinase (uPA, urokinase-type plasminogen activator) is over-expressed in certain tumors and is considered to be the strongest single indicator of poor prognosis in patients with metastatic breast cancer. In this article, we describe the isolation and affinity maturation of a fully human recombinant antibody (termed DS2), specific to the human uPA and capable of inhibiting its enzymatic activity with an IC50 value in the low nanomolar range. The novel antibody cross-reacts with murine uPA. It was expressed both as scFv fragment and in IgG format, allowing a systematic comparative immunofluorescence (IF) analysis of the uPA expression patterns in a large panel of human und murine tumors and of normal human tissues. Although uPA was strongly expressed in virtually all tumor specimens tested, it exhibited only a weak expression in certain normal tissues (mainly in the colon, lung, spleen and bone marrow). IgG(DS2) was not able to inhibit cancer growth in immunocompromised mice bearing subcutaneous human MDA-MB-231 or DoHH-2 tumors. However, an ex vivo IF analysis confirmed the ability of the DS2 antibody to preferentially localize at the tumor site compared with normal organs. Collectively, these data suggest that uPA blocking antibodies may not be indicated for cancer growth inhibition strategies, but may serve as valuable tools for the implementation of pharmacodelivery strategies against a variety of different tumors