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Analysis of a Streptomyces coelicolor A3(2) locus containing the nucleoside diphosphate kinase (ndk) and folylpolyglutamate synthetase (folC) genes

Burger, Annette ; Brandt, Birgit ; Süsstrunk, Urs ; Thompson, Charles J. ; Wohlleben, Wolfgang

In: FEMS Microbiology Letters, 1998, vol. 159, no. 2, p. 283-291

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    Titre
    • Analysis of a Streptomyces coelicolor A3(2) locus containing the nucleoside diphosphate kinase (ndk) and folylpolyglutamate synthetase (folC) genes
    Auteur
    • Burger, Annette. Maikrobiologie/Biotechnologie, Eberhard-Karls-Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany
    • Brandt, Birgit. Maikrobiologie/Biotechnologie, Eberhard-Karls-Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany
    • Süsstrunk, Urs. Department of Microbiology, Biozentrum, University of Basel, Klingelbergstraße 70, CH-4056 Basel, Switzerland
    • Thompson, Charles J.. Department of Microbiology, Biozentrum, University of Basel, Klingelbergstraße 70, CH-4056 Basel, Switzerland
    • Wohlleben, Wolfgang. Maikrobiologie/Biotechnologie, Eberhard-Karls-Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:294156
    Summary
    A 3.6-kb DNA fragment from Streptomyces coelicolor A3(2) with the genes valS probably encoding a valyl-tRNA synthetase, folC encoding folylpolyglutamate synthetase, and ndk encoding a nucleoside diphosphate kinase was analysed. folC and ndk are separated by a small open reading frame of unknown function, orfX. The deduced folC gene product is a protein of 46 677 Da whose sequence is similar to other folylpolyglutamate synthetases and folylpolyglutamate synthetase-dihydrofolate synthetases from both Gram-positive and Gram-negative bacteria. After cloning folC behind the lacZ promoter, the Streptomyces folC complemented a folC mutant of Escherichia coli. An essential function for Streptomyces folC was suggested by the fact that it could not be mutated using a conventional gene disruption technique