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A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells

Müller, Konrad ; Engesser, Raphael ; Metzger, Stéphanie ; Schulz, Simon ; Kämpf, Michael M. ; Busacker, Moritz ; Steinberg, Thorsten ; Tomakidi, Pascal ; Ehrbar, Martin ; Nagy, Ferenc ; Timmer, Jens ; Zubriggen, Matias D. ; Weber, Wilfried

In: Nucleic Acids Research, 2013, vol. 41, no. 7, p. e77-e77

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    Titre
    • A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells
    Auteur
    • Müller, Konrad. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Engesser, Raphael. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Metzger, Stéphanie. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Schulz, Simon. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Kämpf, Michael M.. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Busacker, Moritz. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Steinberg, Thorsten. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Tomakidi, Pascal. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Ehrbar, Martin. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Nagy, Ferenc. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Timmer, Jens. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Zubriggen, Matias D.. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    • Weber, Wilfried. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Department of Physics, University of Freiburg, Hermann-Herder-strasse. 3,79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Obstetrics, University Hospital Zurich, 8091 Zurich, Switzerland, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland, Department of Oral Biotechnology, University Hospital of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Zurich Center for Integrative Human Physiology, 8057 Zurich, Switzerland, Biological Research Centre, Institute of Plant Biology, 6726 Szeged, Hungary, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany, Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany and Freiburg Initiative in Systems Biology (FRISYS), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Nucleic Acids Research, 2013, vol. 41, no. 7, p. e77-e77. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:294065
    Summary
    Growth and differentiation of multicellular systems is orchestrated by spatially restricted gene expression programs in specialized subpopulations. The targeted manipulation of such processes by synthetic tools with high-spatiotemporal resolution could, therefore, enable a deepened understanding of developmental processes and open new opportunities in tissue engineering. Here, we describe the first red/far-red light-triggered gene switch for mammalian cells for achieving gene expression control in time and space. We show that the system can reversibly be toggled between stable on- and off-states using short light pulses at 660 or 740 nm. Red light-induced gene expression was shown to correlate with the applied photon number and was compatible with different mammalian cell lines, including human primary cells. The light-induced expression kinetics were quantitatively analyzed by a mathematical model. We apply the system for the spatially controlled engineering of angiogenesis in chicken embryos. The system's performance combined with cell- and tissue-compatible regulating red light will enable unprecedented spatiotemporally controlled molecular interventions in mammalian cells, tissues and organisms