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Multi-chromatic control of mammalian gene expression and signaling

Müller, Konrad ; Engesser, Raphael ; Schulz, Simon ; Steinberg, Thorsten ; Tomakidi, Pascal ; Weber, Cornelia C. ; Ulm, Roman ; Timmer, Jens ; Zurbriggen, Matias D. ; Weber, Wilfried

In: Nucleic Acids Research, 2013, vol. 41, no. 12, p. e124-e124

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    Titre
    • Multi-chromatic control of mammalian gene expression and signaling
    Auteur
    • Müller, Konrad. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Physics Department, University of Freiburg, Hermann-Herder-Str. 3, 79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Oral Biotechnology, University Hospital Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Novartis Pharma AG, Biologics Process R&D, CH-4002 Basel, Switzerland, Department of Botany and Plant Biology, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany and Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany
    • Engesser, Raphael. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Physics Department, University of Freiburg, Hermann-Herder-Str. 3, 79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Oral Biotechnology, University Hospital Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Novartis Pharma AG, Biologics Process R&D, CH-4002 Basel, Switzerland, Department of Botany and Plant Biology, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany and Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany
    • Schulz, Simon. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Physics Department, University of Freiburg, Hermann-Herder-Str. 3, 79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Oral Biotechnology, University Hospital Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Novartis Pharma AG, Biologics Process R&D, CH-4002 Basel, Switzerland, Department of Botany and Plant Biology, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany and Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany
    • Steinberg, Thorsten. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Physics Department, University of Freiburg, Hermann-Herder-Str. 3, 79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Oral Biotechnology, University Hospital Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Novartis Pharma AG, Biologics Process R&D, CH-4002 Basel, Switzerland, Department of Botany and Plant Biology, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany and Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany
    • Tomakidi, Pascal. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Physics Department, University of Freiburg, Hermann-Herder-Str. 3, 79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Oral Biotechnology, University Hospital Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Novartis Pharma AG, Biologics Process R&D, CH-4002 Basel, Switzerland, Department of Botany and Plant Biology, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany and Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany
    • Weber, Cornelia C.. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Physics Department, University of Freiburg, Hermann-Herder-Str. 3, 79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Oral Biotechnology, University Hospital Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Novartis Pharma AG, Biologics Process R&D, CH-4002 Basel, Switzerland, Department of Botany and Plant Biology, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany and Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany
    • Ulm, Roman. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Physics Department, University of Freiburg, Hermann-Herder-Str. 3, 79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Oral Biotechnology, University Hospital Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Novartis Pharma AG, Biologics Process R&D, CH-4002 Basel, Switzerland, Department of Botany and Plant Biology, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany and Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany
    • Timmer, Jens. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Physics Department, University of Freiburg, Hermann-Herder-Str. 3, 79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Oral Biotechnology, University Hospital Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Novartis Pharma AG, Biologics Process R&D, CH-4002 Basel, Switzerland, Department of Botany and Plant Biology, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany and Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany
    • Zurbriggen, Matias D.. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Physics Department, University of Freiburg, Hermann-Herder-Str. 3, 79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Oral Biotechnology, University Hospital Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Novartis Pharma AG, Biologics Process R&D, CH-4002 Basel, Switzerland, Department of Botany and Plant Biology, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany and Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany
    • Weber, Wilfried. Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany, Physics Department, University of Freiburg, Hermann-Herder-Str. 3, 79104 Freiburg, Germany, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany, Department of Oral Biotechnology, University Hospital Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany, Novartis Pharma AG, Biologics Process R&D, CH-4002 Basel, Switzerland, Department of Botany and Plant Biology, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland, Freiburg Centre for Biosystems Analysis (ZBSA), University of Freiburg, Habsburgerstrasse 49, 79104 Freiburg, Germany and Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Albertstrasse 19, 79104 Freiburg, Germany
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Nucleic Acids Research, 2013, vol. 41, no. 12, p. e124-e124. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:293172
    Summary
    The emergence and future of mammalian synthetic biology depends on technologies for orchestrating and custom tailoring complementary gene expression and signaling processes in a predictable manner. Here, we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different wavelengths. To this end, we developed an ultraviolet B (UVB)-inducible expression system by designing a UVB-responsive split transcription factor based on the Arabidopsis thaliana UVB receptor UVR8 and the WD40 domain of COP1. The system allowed high (up to 800-fold) UVB-induced gene expression in human, monkey, hamster and mouse cells. Based on a quantitative model, we determined critical system parameters. By combining this UVB-responsive system with blue and red light-inducible gene control technology, we demonstrate multi-chromatic multi-gene control by differentially expressing three genes in a single cell culture in mammalian cells, and we apply this system for the multi-chromatic control of angiogenic signaling processes. This portfolio of optogenetic tools enables the design and implementation of synthetic biological networks showing unmatched spatiotemporal precision for future research and biomedical applications