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Fibronectin-Cleaving Activity in Bronchial Secretions of Patients with Cystic Fibrosis

Suter, Susanne ; Schaad, U. B. ; Morgenthaler, J. J. ; Chevallier, I. ; Schnebli, H. P.

In: Journal of Infectious Diseases, 1988, vol. 158, no. 1, p. 89-100

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    Titre
    • Fibronectin-Cleaving Activity in Bronchial Secretions of Patients with Cystic Fibrosis
    Auteur
    • Suter, Susanne. Department of Pediatrics, Children's Hospital, University of Geneva, Geneva; the Department of Pediatrics, Children's Hospital, University of Bern, and the Central Laboratory, Swiss Red Cross, Bern, Basel, Switzerland
    • Schaad, U. B.. Department of Pediatrics, Children's Hospital, University of Geneva, Geneva; the Department of Pediatrics, Children's Hospital, University of Bern, and the Central Laboratory, Swiss Red Cross, Bern, Basel, Switzerland
    • Morgenthaler, J. J.. Department of Pediatrics, Children's Hospital, University of Geneva, Geneva; the Department of Pediatrics, Children's Hospital, University of Bern, and the Central Laboratory, Swiss Red Cross, Bern, Basel, Switzerland
    • Chevallier, I.. Department of Pediatrics, Children's Hospital, University of Geneva, Geneva; the Department of Pediatrics, Children's Hospital, University of Bern, and the Central Laboratory, Swiss Red Cross, Bern, Basel, Switzerland
    • Schnebli, H. P.. Department of Pediatrics, Children's Hospital, University of Geneva, Geneva; the Department of Pediatrics, Children's Hospital, University of Bern, and the Central Laboratory, Swiss Red Cross, Bern, Basel, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Journal of Infectious Diseases, 1988, vol. 158, no. 1, p. 89-100. The University Chicago Press
    Autre version électronique
    Classification
    • Santé
    Identifiant OAI-PMH
    • oai:doc.rero.ch:292947
    Summary
    In cystic fibrosis, colonization of the airways with Pseudomonas aeruginosa follows colonization with Staphylococcus aureus and is related to accelerated deterioration of pulmonary function. Because P. aeruginosa adheres better to cell surfaces devoid of fibronectin, we searched for fibronectin-cleaving activity in bronchial secretions and saliva from 24 patients with cystic fibrosis who were followed up for 4.5 y and from two control groups. Proteolytic activity against 125I-labeled fibronectin wascontinuously present in cystic fibrosis bronchial secretions; significantly higher fibronectin-cleaving activity was found in older vs. younger patients, in patients with advanced disease stages determined by a five-stage scoring system, and in those colonized with P. aeruginosa. The fibronectin-cleaving activity was due to neutrophil elastase and cathepsin G. Cystic fibrosis bronchial secretions had proteolytic activity against surface fibronectin of airway mucosal cells. Thus fibronectin-cleaving activity of bronchial secretions rather than of saliva may favor P. aeruginosa colonization of the upper respiratory tract in individuals with cystic fibrosis