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Transcriptional Repression by RING Finger Protein TIF1β That Interacts with the KRAB Repressor Domain of KOX1

Moosmann, Peter ; Georgiev, Oleg ; Le Douarin, Bertrand ; Bourquin, Jean-Pierre ; Schaffner, Walter

In: Nucleic Acids Research, 1996, vol. 24, no. 24, p. 4859-4867

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    Titre
    • Transcriptional Repression by RING Finger Protein TIF1β That Interacts with the KRAB Repressor Domain of KOX1
    Auteur
    • Moosmann, Peter. Institut für Molekularbiologie der Universität, Abteilung II, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland
    • Georgiev, Oleg. Institut für Molekularbiologie der Universität, Abteilung II, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland
    • Le Douarin, Bertrand. Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Collège de France, BP 163, 67404 Illkirch cedex, France
    • Bourquin, Jean-Pierre. Institut für Molekularbiologie der Universität, Abteilung II, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland
    • Schaffner, Walter. Institut für Molekularbiologie der Universität, Abteilung II, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Nucleic Acids Research, 1996, vol. 24, no. 24, p. 4859-4867. Oxford University Press
    Autre version électronique
    Classification
    • Santé
    Identifiant OAI-PMH
    • oai:doc.rero.ch:290435
    Summary
    Many of the vertebrate zinc finger factors of the Krüppel type (C2H2 zinc fingers) contain in their N-terminus a conserved sequence referred to as the KRAB (Krüppel-associated box) domain that, when tethered to DNA, efficiently represses transcription. Using the yeast two-hybrid system, we have isolated an 835 amino acid RING finger (C3HC4 zinc finger) protein, TIF1β (also named KAP-1), that specifically interacts with the KRAB domain of the human zinc finger factor KOX1/ZNF10. TIF1β, TIF1α, PML and efp belong to a characteristic subgroup of RING finger proteins that contain one or two other Cys/His-rich clusters (B boxes) and a putative coiled-coil in addition to the classical C3HC4 RING finger motif (RBCC configuration). Like TIF1α, TIF1β also contains an additional Cys/His cluster (PHD finger) and a bromorelated domain. When tethered to DNA, TIF1β can repress transcription in transiently transfected mammalian cells both from promoter-proximal and remote (enhancer) positions, similarly to the KRAB domain itself. We propose that TIF1β is a mediator of the transcriptional repression exerted by the KRAB domain