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Comparison of carbohydrate utilization in man using indirect calorimetry and mass spectrometry after an oral load of 100 g naturally-labelled [13C]glucose

Ebiner, J. R. ; Acheson, K. J. ; Doerner, A. ; Maeder, E. ; Arnaud, M. J. ; Jéquier, E. ; Felber, J. P.

In: British Journal of Nutrition, 1979, vol. 41, no. 3, p. 419-429

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    Titre
    • Comparison of carbohydrate utilization in man using indirect calorimetry and mass spectrometry after an oral load of 100 g naturally-labelled [13C]glucose
    Auteur
    • Ebiner, J. R.. Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
    • Acheson, K. J.. Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
    • Doerner, A.. Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
    • Maeder, E.. Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
    • Arnaud, M. J.. Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
    • Jéquier, E.. Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
    • Felber, J. P.. Divisions de Biochimie Clinique et de Physiologie Clinique, Département de Médecine, CHUV, 1011 Lausanne, Switzerland, and Laboratoire Nestlé, 1814 La-Tour-de-Peilz, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • British Journal of Nutrition, 1979, vol. 41, no. 3, p. 419-429. Cambridge University Press
    Autre version électronique
    Classification
    • Santé
    Identifiant OAI-PMH
    • oai:doc.rero.ch:289624
    Summary
    1. Carbohydrate (CHO) oxidation was measured simultaneously in a group of five normal subjects after an oral load of 100 g naturally-labelled [13C]glucose, using indirect calorimetry and mass spectrometry. 2. CHO utilization, calculated from the results of indirect calorimetry, increased 30 min after the glucose load to reach a peak at 90 min. It then decreased to reach basal values at 380 min. Cumulative total CHO oxidation at 480 min was 83±8g, and CHO oxidized above basal levels, 37±3 g. 3. Enrichment of expired carbon dioxide with 13C began at 60 min and maximum values were observed at 270 min. At 480 min, cumulative CHO oxidation measured by use of [13C]glucose was 29 g. The difference from calorimetric values can be attributed in part to the slow isotopic dilution in the glucose and bicarbonate pools. 4. Thus, approximately 30% of the glucose load was oxidized during the 8 h after its ingestion and this accounts for a significant part of the increased CHO oxidation (37 g), as measured by indirect calorimetry