Université de Fribourg

Mature lipid droplets are accessible to ER luminal proteins

Mishra, Shirish ; Khaddaj, Rasha ; Cottier, Stéphanie ; Stradalova, Vendula ; Jacob, Claire ; Schneiter, Roger

In: Journal of Cell Science, 2016, vol. 129, no. 20, p. 3803–3815

Lipid droplets are found in most organisms where they serve to store energy in the form of neutral lipids. They are formed at the endoplasmic reticulum (ER) membrane where the neutral-lipid-synthesizing enzymes are located. Recent results indicate that lipid droplets remain functionally connected to the ER membrane in yeast and mammalian cells to allow the exchange of both lipids and integral...

Université de Fribourg

Acyl-CoA-binding protein, Acb1p, is required for normal vacuole function and ceramide synthesis in Saccharomyces cerevisiae

Faergeman, Nils J. ; Feddersen, Søren ; Christiansen, Janne K. ; Larsen, Morten K. ; Schneiter, Roger ; Ungermann, Christian ; Mutenda, Kudzai ; Roepstroff, Peter ; Knudsen, Jens

In: Biochemical Journal, 2004, vol. 380, p. 907-918

In the present study, we show that depletion of acyl-CoA-binding protein, Acb1p, in yeast affects ceramide levels, protein trafficking, vacuole fusion and structure. Vacuoles in Acb1p-depleted cells are multi-lobed, contain significantly less of the SNAREs (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors) Nyv1p, Vam3p and Vti1p, and are unable to fuse in vitro. Mass...