Université de Fribourg

Ubiquitin release from eL40 is required for cytoplasmic maturation and function of 60S ribosomal subunits in Saccharomyces cerevisiae

Martín‐Villanueva, Sara ; Fernández‐Pevida, Antonio ; Fernández‐Fernández, José ; Kressler, Dieter ; Cruz, Jesús de la

In: The FEBS Journal, 2019, vol. 0, no. 0, p. -

Ubiquitin is generated by proteolytic cleavage of precursor proteins in which it is fused either to itself, constituting a linear polyubiquitin protein of head‐to‐tail monomers, or as a single N‐terminal moiety to one of two ribosomal proteins, eL40 (Ubi1/2 precursors) and eS31 (Ubi3 precursor). It has been proposed that the ubiquitin moiety fused to these ribosomal proteins could act...

Université de Fribourg

The eukaryote-specific N-terminal extension of ribosomal protein S31 contributes to the assembly and function of 40S ribosomal subunits

Fernández-Pevida, Antonio ; Martín-Villanueva, Sara ; Murat, Guillaume ; Lacombe, Thierry ; Kressler, Dieter ; Cruz, Jesús de la

In: Nucleic Acids Research, 2016, vol. 44, no. 16, p. 7777–7791

The archaea-/eukaryote-specific 40S-ribosomal-subunit protein S31 is expressed as an ubiquitin fusion protein in eukaryotes and consists of a conserved body and a eukaryote-specific N-terminal extension. In yeast, S31 is a practically essential protein, which is required for cytoplasmic 20S pre-rRNA maturation. Here, we have studied the role of the N-terminal extension of the yeast S31...

Université de Fribourg

Final Pre-40S Maturation Depends on the Functional Integrity of the 60S Subunit Ribosomal Protein L3

García-Gómez, Juan J. ; Fernández-Pevida, Antonio ; Lebaron, Simon ; Rosado, Iván V. ; Tollervey, David ; Kressler, Dieter ; Cruz, Jesús de la

In: PLoS Genetics, 2014, vol. 10, no. 3, p. e1004205

Ribosomal protein L3 is an evolutionarily conserved protein that participates in the assembly of early pre-60S particles. We report that the rpl3[W255C] allele, which affects the affinity and function of translation elongation factors, impairs cytoplasmic maturation of 20S pre-rRNA. This was not seen for other mutations in or depletion of L3 or other 60S ribosomal proteins. Surprisingly, pre-40S...

Université de Fribourg

Yeast ribosomal protein L40 assembles late into pre-60S ribosomes and is required for their cytoplasmic maturation

Fernandez-Pevida, Antonio ; Rodriguez-Galan, Olga ; Diaz-Quintana, Antonio ; Kressler, Dieter ; Cruz, Jesus de la

In: Journal of Biological Chemistry, 2012, p. -

Most ribosomal proteins play important roles in ribosome biogenesis and function. Herein, we have examined the contribution of the essential ribosomal protein L40 in these processes in the yeast Saccharomyces cerevisiae. Deletion of either the RPL40A or RPL40B gene and in vivo depletion of L40 impair 60S ribosomal subunit biogenesis. Polysome profile analyses reveal the accumulation of half-mers...

Université de Fribourg

The power of AAA-ATPases on the road of pre-60S ribosome maturation — Molecular machines that strip pre-ribosomal particles

Kressler, Dieter ; Hurt, Ed ; Bergler, Helmut ; Baßler, Jochen

In: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2011, p. -

The biogenesis of ribosomes is a fundamental cellular process, which provides the molecular machines that synthesize all cellular proteins. The assembly of eukaryotic ribosomes is a highly complex multi-step process that requires more than 200 ribosome biogenesis factors, which mediate a broad spectrum of maturation reactions. The participation of many energy-consuming enzymes (e.g. AAA-type...