Université de Fribourg

Ubiquitin release from eL40 is required for cytoplasmic maturation and function of 60S ribosomal subunits in Saccharomyces cerevisiae

Martín‐Villanueva, Sara ; Fernández‐Pevida, Antonio ; Fernández‐Fernández, José ; Kressler, Dieter ; Cruz, Jesús de la

In: The FEBS Journal, 2019, vol. 0, no. 0, p. -

Ubiquitin is generated by proteolytic cleavage of precursor proteins in which it is fused either to itself, constituting a linear polyubiquitin protein of head‐to‐tail monomers, or as a single N‐terminal moiety to one of two ribosomal proteins, eL40 (Ubi1/2 precursors) and eS31 (Ubi3 precursor). It has been proposed that the ubiquitin moiety fused to these ribosomal proteins could act...

Université de Fribourg

The eukaryote-specific N-terminal extension of ribosomal protein S31 contributes to the assembly and function of 40S ribosomal subunits

Fernández-Pevida, Antonio ; Martín-Villanueva, Sara ; Murat, Guillaume ; Lacombe, Thierry ; Kressler, Dieter ; Cruz, Jesús de la

In: Nucleic Acids Research, 2016, vol. 44, no. 16, p. 7777–7791

The archaea-/eukaryote-specific 40S-ribosomal-subunit protein S31 is expressed as an ubiquitin fusion protein in eukaryotes and consists of a conserved body and a eukaryote-specific N-terminal extension. In yeast, S31 is a practically essential protein, which is required for cytoplasmic 20S pre-rRNA maturation. Here, we have studied the role of the N-terminal extension of the yeast S31...

Université de Fribourg

Final Pre-40S Maturation Depends on the Functional Integrity of the 60S Subunit Ribosomal Protein L3

García-Gómez, Juan J. ; Fernández-Pevida, Antonio ; Lebaron, Simon ; Rosado, Iván V. ; Tollervey, David ; Kressler, Dieter ; Cruz, Jesús de la

In: PLoS Genetics, 2014, vol. 10, no. 3, p. e1004205

Ribosomal protein L3 is an evolutionarily conserved protein that participates in the assembly of early pre-60S particles. We report that the rpl3[W255C] allele, which affects the affinity and function of translation elongation factors, impairs cytoplasmic maturation of 20S pre-rRNA. This was not seen for other mutations in or depletion of L3 or other 60S ribosomal proteins. Surprisingly, pre-40S...