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Université de Fribourg

Rapid detection of carbapenemase-producing Pseudomonas spp. using the NitroSpeed-Carba NP test

Sadek, Mustafa ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2021, vol. 99, no. 3, p. 115280

The NitroSpeed-Carba NP test was used to rapidly detect and discriminate between the different types of carbapenemases (classes A, B, and D) within 30 minutes among a collection of 202 Pseudomonas sp. strains (mostly Pseudomonas aeruginosa). A total of 99 carbapenemase–(including enzymes exhibiting weak carbapenemase activity such as several Guyana Extended-Spectrum (GES)-ß-lactamases) and...

Université de Fribourg

Cross-border emergence of clonal lineages of ST38 Escherichia coli producing the OXA-48-like carbapenemase OXA-244 in Germany and Switzerland

Falgenhauer, Linda ; Nordmann, Patrice ; Imirzalioglu, Can ; Yao, Yancheng ; Falgenhauer, Jane ; M.Hauri, Anja ; Heinmüller, Petra ; Chakraborty, Trinad

In: International Journal of Antimicrobial Agents, 2020, vol. 56, no. 6, p. 106157

Background: Carbapenemase-producing Gram-negative bacteria cause infections that are difficult to treat and represent a rising threat to healthcare systems worldwide. This study analysed isolates of Escherichia coli (E. coli), a species associated with nosocomial-acquired and community-acquired infections, from hospitals in Germany and Switzerland exhibiting a slight decrease in...

Université de Fribourg

Cross-border emergence of Escherichia coli producing the carbapenemase NDM-5 in Switzerland and Germany

Chakraborty, Trinad ; Sadek, Mustafa ; Yao, Yancheng ; Imirzalioglu, Can ; Stephan, Roger ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2020, p. -

A series of clinical NDM-5-producing E. coli isolates obtained from two surveillance networks of carbapenem-producing Enterobacterales from 2018-2019, namely Switzerland (NARA) and Germany (SurvCARE), were analyzed. The 33 NDM-5- producing E. coli isolates were highly resistant to β-lactams including the novel ß- lactam/ß-lactamase inhibitor combinations (ceftazidime/avibactam, ...

Université de Fribourg

Rapid ESBL NP test for rapid detection of expanded-spectrum β-lactamase producers in enterobacterales

Demord, Anthony ; Poirel, Laurent ; D'Emidio, Fernando ; Pomponio, Stefano ; Nordmann, Patrice

In: Microbial Drug Resistance, 2020, p. -

Objectives: To evaluate the biochemical Rapid ESBL NP® (Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales.Methods: A total of 100 clinical gram-negative strains (40 ESBL producers with or without cephalosporinase, 43 carbapenemase producers with or without additional ESBLs, 8 AmpC-type producers, 6 penicillinase producers, and 3 ...