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Université de Fribourg

Effects of the poly(ADP-ribose) polymerase inhibitor olaparib in cerulein-induced pancreatitis

Ahmad, Akbar ; Haas De Mello, Aline ; Szczesny, Bartosz ; Törö, Gábor ; Marcatti, Michela ; Druzhyna, Nadiya ; Liaudet, Lucas ; Tarantini, Stefano ; Salomao, Reinaldo ; Garcia Soriano, Francisco ; Szabo, Csaba

In: Shock, 2020, vol. 53, no. 5, p. 653–665

Objective: Activation of the constitutive nuclear and mitochondrial enzyme poly (ADP- ribose) polymerase (PARP) has been implicated in the pathogenesis of cell dysfunction, inflammation, and organ failure in various forms of critical illness. The objective of our study was to evaluate the efficacy and safety of the clinically approved PARP inhibitor olaparib in an experimental model of...

Université de Fribourg

An inflamed human alveolar model for testing the efficiency of anti-inflammatory drugs in vitro

Drasler, Barbara ; Karakocak, Bedia Begum ; Tankus, Esma Bahar ; Barosova, Hana ; Abe, Jun ; Sousa de Almeida, Mauro ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Frontiers in Bioengineering and Biotechnology, 2020, vol. 8, p. -

A large number of prevalent lung diseases is associated with tissue inflammation. Clinically, corticosteroid therapies are applied systemically or via inhalation for the treatment of lung inflammation, and a number of novel therapies are being developed that require preclinical testing. In alveoli, macrophages and dendritic cells play a key role in initiating and diminishing pro-inflammatory...

Université de Fribourg

A novel 3D intestine barrier model to study the immune response upon exposure to microplastics

Lehner, Roman ; Wohlleben, Wendel ; Septiadi, Dedy ; Landsiedel, Robert ; Petri‑Fink, Alke ; Rothen‑Rutishauser, Barbara

In: Archives of Toxicology, 2020, p. -

The plausibility of human exposure to microplastics has increased within the last years. Microplastics have been found in different food types including seafood, salt, sugar and beverages. So far, human health effects of microplastics after ingestion are unknown. Herein, we designed a novel, three- dimensional in vitro intestinal model consisting of the human intestinal epithelial cell lines...

Università della Svizzera italiana

Bovine lactoferrin-induced CCL1 expression involves distinct receptors in monocyte-derived dendritic cells and their monocyte precursors

Latorre, Daniela ; Pulvirenti, Nadia ; Covino, Daniela Angela ; Varano, Barbara ; Purificato, Cristina ; Rainaldi, Gabriella ; Gauzzi, Maria Cristina ; Fantuzzi, Laura ; Conti, Lucia ; Donninelli, Gloria ; Del Cornò, Manuela ; Sabbatucci, Michela ; Gessani, Sandra ; Puddu, Patrizia

In: Toxins, 2015, vol. 7, no. 12, p. 5472-5483

Lactoferrin (LF) exhibits a wide range of immunomodulatory activities including modulation of cytokine and chemokine secretion. In this study, we demonstrate that bovine LF (bLF) up-modulates, in a concentration- and time-dependent manner, CCL1 secretion in monocytes (Mo) at the early stage of differentiation toward dendritic cells (DCs), and in fully differentiated immature Mo-derived DCs...

Università della Svizzera italiana

Macrophage death following influenza vaccination initiates the inflammatory response that promotes dendritic cell function in the draining lymph node

Chatziandreou, Nikolaos ; Farsakoglu, Yagmur ; Palomino-Segura, Miguel ; D’Antuono, Rocco ; Pizzagalli, Diego Ulisse ; Sallusto, Federica ; Lukacs-Kornek, Veronika ; Uguccioni, Mariagrazia ; Corti, Davide ; Turley, Shannon J. ; Lanzavecchia, Antonio ; Carroll, Michael C. ; Gonzalez, Santiago F.

In: Cell reports, 2017, vol. 18, no. 10, p. 2427-2440

The mechanism by which inflammation influences the adaptive response to vaccines is not fully understood. Here, we examine the role of lymph node macrophages (LNMs) in the induction of the cytokine storm triggered by inactivated influenza virus vaccine. Following vaccination, LNMs undergo inflammasome-independent necrosis-like death that is reliant on MyD88 and Toll-like receptor 7 (TLR7)...

Université de Fribourg

Polymer-coated gold nanospheres do not impair the innate immune function of human b lymphocytes in vitro

Hočevar, Sandra ; Milošević, Ana ; Rodriguez-Lorenzo, Laura ; Ackermann-Hirschi, Liliane ; Mottas, Inès ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara ; Bourquin, Carole ; Clift, Martin James David

In: ACS Nano, 2019, vol. 13, no. 6, p. 6790–6800

Gold nanoparticles (GNPs) are intended for use within a variety of biomedical applications due to their physicochemical properties. Although, in general, biocompatibility of GNPs with immune cells such as macrophages and dendritic cells is well established, the impact of GNPs on B lymphocyte immune function remains to be determined. Since B lymphocytes play an important role in health and...

Université de Fribourg

Development of resiquimod-loaded modified PLA-based nanoparticles for cancer immunotherapy: A kinetic study

Thauvin, Cédric ; Widmer, Jérôme ; Mottas, Inès ; Hocevar, Sandra ; Allémann, Eric ; Bourquin, Carole ; Delie, Florence

In: European Journal of Pharmaceutics and Biopharmaceutics, 2019, vol. 139, p. 253–261

Resiquimod (R848), a member of the imidazoquinoline family, is a Toll-like receptor 7/8 agonist with high potency for cancer immunotherapy. However, tolerance induction and adverse effects limit its development as a drug. Encapsulation in a polymer matrix can circumvent these limitations, as shown in our formerly published approach where R848 was loaded into polylactic acid (PLA)-based...