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Université de Fribourg

NGS-based S. aureus typing and outbreak analysis in clinical microbiology laboratories : Lessons learned from a Swiss-wide proficiency test

Dylus, David ; Pillonel, Trestan ; Opota, Onya ; Wüthrich, Daniel ; Seth-Smith, Helena M. B. ; Egli, Adrian ; Leo, Stefano ; Lazarevic, Vladimir ; Schrenzel, Jacques ; Laurent, Sacha ; Bertelli, Claire ; Blanc, Dominique S. ; Neuenschwander, Stefan ; Ramette, Alban ; Falquet, Laurent ; Imkamp, Frank ; Keller, Peter M. ; Kahles, Andre ; Oberhaensli, Simone ; Barbié, Valérie ; Dessimoz, Christophe ; Greub, Gilbert ; Lebrand, Aitana

In: Frontiers in Microbiology, 2020, vol. 11, p. 591093

Whole genome sequencing (WGS) enables high resolution typing of bacteria up to the single nucleotide polymorphism (SNP) level. WGS is used in clinical microbiology laboratories for infection control, molecular surveillance and outbreak analyses. Given the large palette of WGS reagents and bioinformatics tools, the Swiss clinical bacteriology community decided to conduct a ring trial (RT) to...

Université de Fribourg

Tissue-specific isoforms of the single C. elegans Ryanodine receptor gene unc-68 control specific functions

Marques, Filipe ; Thapliyal, Saurabh ; Javer, Avelino ; Shrestha, Priyanka ; Brown, André E. X. ; Glauser, Dominique A.

In: PLOS Genetics, 2020, vol. 16, no. 10, p. e1009102

Ryanodine receptors (RyR) are essential regulators of cellular calcium homeostasis and signaling. Vertebrate genomes contain multiple RyR gene isoforms, expressed in different tissues and executing different functions. In contrast, invertebrate genomes contain a single RyR-encoding gene and it has long been proposed that different transcripts generated by alternative splicing may diversify...

Université de Fribourg

An ATP-dependent partner switch links flagellar C-ring assembly with gene expression

Blagotinsek, Vitan ; Schwan, Meike ; Steinchen, Wieland ; Mrusek, Devid ; Hook, John C. ; Rossmann, Florian ; Freibert, Sven A. ; Kratzat, Hanna ; Murat, Guillaume ; Kressler, Dieter ; Beckmann, Roland ; Beeby, Morgan ; Thormann, Kai M. ; Bange, Gert

In: Proceedings of the National Academy of Sciences, 2020, vol. 117, no. 34, p. 20826–20835

Bacterial flagella differ in their number and spatial arrangement. In many species, the MinD-type ATPase FlhG (also YlxH/FleN) is central to the numerical control of bacterial flagella, and its deletion in polarly flagellated bacteria typically leads to hyperflagellation. The molecular mechanism underlying this numerical control, however, remains enigmatic. Using the model species Shewanella...

Université de Fribourg

Parasite‐driven replacement of a sexual by a closely related asexual taxon in nature

Lohr, Jennifer N. ; Haag, Christoph R.

In: Ecology, 2020, vol. 101, no. 10, p. -

Asexual species are thought to suffer more from coevolving parasites than related sexuals. Yet a variety of studies do not find the patterns predicted by theory. Here, to shine light on this conundrum, we investigate one such case of an asexual advantage in the presence of parasites. We follow the frequency dynamics of sexual and asexual Daphnia pulex in a natural pond that was initially...