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Understanding the fabric of protein crystals: computational classification of biological interfaces and crystal contacts

Capitani, Guido ; Duarte, Jose M. ; Baskaran, Kumaran ; Bliven, Spencer ; Somody, Joseph C.

In: Bioinformatics, 2016, vol. 32, no. 4, p. 481-489

Structure and function analysis of an antibody recognizing all influenza A subtypes

Kallewaard, Nicole L. ; Corti, Davide ; Collins, Patrick J. ; Neu, Ursula ; McAuliffe, Josephine M. ; Benjamin, Ebony ; Wachter-Rosati, Leslie ; Palmer-Hill, Frances J. ; Yuan, Andy Q. ; Walker, Philip A. ; Vorlaender, Matthias K. ; Bianchi, Siro ; Guarino, Barbara ; De Marco, Anna ; Vanzetta, Fabrizia ; Agatic, Gloria ; Foglierini, Mathilde ; Pinna, Debora ; Fernandez-Rodriguez, Blanca ; Fruehwirth, Alexander ; Silacci, Chiara ; Ogrodowicz, Roksana W. ; Martin, Stephen R. ; Sallusto, Federica ; Suzich, JoAnn A. ; Lanzavecchia, Antonio ; Zhu, Qing ; Gamblin, Steven J. ; Skehel, John J.

In: Cell, 2016, vol. 166, no. 3, p. 596-608

Influenza virus remains a threat because of its ability to evade vaccine-induced immune responses due to antigenic drift. Here, we describe the isolation, evolution, and structure of a broad-spectrum human monoclonal antibody (mAb), MEDI8852, effectively reacting with all influenza A hemagglutinin (HA) subtypes. MEDI8852 uses the heavy-chain VH6-1 gene and has higher potency and breadth when...

Rational engineering of a human anti-dengue antibody through experimentally validated computational docking

Simonelli, Luca ; Pedotti, Mattia ; Beltramello, Martina ; Livoti, Elsa ; Calzolai, Luigi ; Sallusto, Federica ; Lanzavecchia, Antonio ; Varani, Luca

In: Plos one, 2013, vol. 8, no. 2, p. e55561

Antibodies play an increasing pivotal role in both basic research and the biopharmaceutical sector, therefore technology for characterizing and improving their properties through rational engineering is desirable. This is a difficult task thought to require high-resolution x-ray structures, which are not always available. We, instead, use a combination of solution NMR epitope mapping and ...

Chronic delivery of antibody fragments using immunoisolated cell implants as a passive vaccination tool

Belaunzaran, Osiris Marroquin ; Cordero, Maria Isabel ; Setola, Veronica ; Bianchi, Siro ; Galli, Carmela ; Bouche, Nicolas ; Mlynarik, Vladimir ; Gruetter, Rolf ; Sandi, Carmen ; Bensadoun, Jean-Charles ; Molinari, Maurizio ; Aebischer, Patrick

In: Plos one, 2011, vol. 6, no. 4, p. e18268

Background: Monoclonal antibodies and antibody fragments are powerful biotherapeutics for various debilitating diseases. However, high production costs, functional limitations such as inadequate pharmacokinetics and tissue accessibility are the current principal disadvantages for broadening their use in clinic. Methodology and Principal Findings: We report a novel method for the long-term ...

Crystal structure and size-dependent neutralization properties of HK20, a human monoclonal antibody binding to the highly conserved heptad repeat 1 of gp41

Sabin, Charles ; Corti, Davide ; Buzon, Victor ; Seaman, Mike S. ; Hulsik, David Lutje ; Hinz, Andreas ; Vanzetta, Fabrizia ; Agatic, Gloria ; Silacci, Chiara ; Mainetti, Lara ; Scarlatti, Gabriella ; Sallusto, Federica ; Weiss, Robin ; Lanzavecchia, Antonio ; Weissenhorn, Winfried

In: Plos pathogens, 2010, vol. 6, no. 11, p. e1001195

The human monoclonal antibody (mAb) HK20 neutralizes a broad spectrum of primary HIV-1 isolates by targeting the highly conserved heptad repeat 1 (HR1) of gp41, which is transiently exposed during HIV-1 entry. Here we present the crystal structure of the HK20 Fab in complex with a gp41 mimetic 5-Helix at 2.3 A° resolution. HK20 employs its heavy chain CDR H2 and H3 loops to bind into a...

Erratum to: Quantifying Protein-Ligand Binding Constants Using Electrospray Ionization Mass Spectrometry: A Systematic Binding Affinity Study of a Series of Hydrophobically Modified Trypsin Inhibitors

Cubrilovic, Dragana ; Biela, Adam ; Sielaff, Frank ; Steinmetzer, Torsten ; Klebe, Gerhard ; Zenobi, Renato

In: Journal of The American Society for Mass Spectrometry, 2014, vol. 25, no. 7, p. 1305-1305

CD1d-antibody fusion proteins target iNKT cells to the tumor and trigger long-term therapeutic responses

Corgnac, Stéphanie ; Perret, Rachel ; Derré, Laurent ; Zhang, Lianjun ; Stirnemann, Kathrin ; Zauderer, Maurice ; Speiser, Daniel ; Mach, Jean-Pierre ; Romero, Pedro ; Donda, Alena

In: Cancer Immunology, Immunotherapy, 2013, vol. 62, no. 4, p. 747-760

Detection and Analysis of Protein Aggregation with Confocal Single Molecule Fluorescence Spectroscopy

Hillger, Frank ; Nettels, Daniel ; Dorsch, Simone ; Schuler, Benjamin

In: Journal of Fluorescence, 2007, vol. 17, no. 6, p. 759-765

Quantifying Protein-Ligand Binding Constants using Electrospray Ionization Mass Spectrometry: A Systematic Binding Affinity Study of a Series of Hydrophobically Modified Trypsin Inhibitors

Cubrilovic, Dragana ; Biela, Adam ; Sielaff, Frank ; Steinmetzer, Torsten ; Klebe, Gerhard ; Zenobi, Renato

In: Journal of The American Society for Mass Spectrometry, 2012, vol. 23, no. 10, p. 1768-1777

Breaking the Aggregation of the Monoclonal Antibody Bevacizumab (Avastin®) by Dexamethasone Phosphate: Insights from Molecular Modelling and Asymmetrical Flow Field-Flow Fractionation

Veurink, Marieke ; Westermaier, Yvonne ; Gurny, Robert ; Scapozza, Leonardo

In: Pharmaceutical Research, 2013, vol. 30, no. 4, p. 1176-1187