Mémoire de diplôme HES : Haute Ecole d'Ingénierie, 2007.
The emergence of several pathogenic bacteria and the adaptation of part of those resistant to antibiotics currently used, in clinically treatments, needs the research for new antibiotic development and the creation of a “Superhost”, allowing a large scale production of antibiotics. Streptomyces belongs to Actinomycetes , a group of gram-positive bacteria, producing more than 50% of known...
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Mémoire de diplôme HES : Haute Ecole d'Ingénierie, 2007.
Aim: Confirm the presence of pedA gene, known part of pediocin PD-1 operon, in one of Pediococcus damnosus plasmids. Construct a plasmid library using this isolated plasmid, digested with endonucleases and subcloned in pZErO-2 vector. Identify recombinant plasmids containing pedA gene by PCR screening or/and by hybridization with pedA probe. Sequence the insert and localize pedA region in order...
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In: Bioinformatics, 2007, vol. 23, no. 23, p. 3185-3192
Motivation: Regulatory gene networks contain generic modules such as feedback loops that are essential for the regulation of many biological functions. The study of the stochastic mechanisms of gene regulation is instrumental for the understanding of how cells maintain their expression at levels commensurate with their biological role, as well as to engineer gene expression switches of...
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In: Journal of Evolutionary Biology, 2008///doi:10.1111/j.1420-9101.2008.01541.x
Although there is little doubt that hosts evolve to reduce parasite damage, little is known about the evolutionary time scale on which host populations may adapt under natural conditions. Here we study the effects of selection by the microsporidian parasite Octosporea bayeri on populations of Daphnia magna. In a field study, we infected replicated populations of D. magna with the parasite,...
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In: Plant Physiology, 2009, p. -
Salicylic acid (SA) is an important mediator of plant defense response. In Arabidopsis thaliana, this compound was proposed to derive mainly from isochorismate, itself produced from chorismate through the activity of ICS1 (Isochorismate Synthase1). Null ics1 mutants still accumulate some SA, suggesting the existence of an enzymatic activity redundant with ICS1 or of an alternative...
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In: Physical Review A, 2007, vol. 76, no. 2, p. 022502
We present a systematic experimental study of the absorption and fluorescence spectra of the 6S1/2-6P1/2 transition in Cs atoms isolated in solid ⁴He matrices. The bubble model developed earlier for alkali-metal atoms in liquid He is revised and applied to the present system. The analysis of the dependencies of absorption and fluorescence wavelengths on He...
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In: Molecular and Cellular Biology, 2007, vol. 27, no. 19, p. 6876-6888
The pentatricopeptide repeat (PPR), a degenerate 35-amino-acid motif, defines a novel eukaryotic protein family. Plants have 400 to 500 distinct PPR proteins, whereas other eukaryotes generally have fewer than 5. The few PPR proteins that have been studied have roles in organellar gene expression, probably via direct interaction with RNA. Here we show that the parasitic protozoan Trypanosoma...
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In: PLoS ONE, 2007, vol. 2, no. 5, p. e488
Bax-induced permeabilization of the mitochondrial outer membrane and release of cytochrome c are key events in apoptosis. Although Bax can compromise mitochondria in primitive unicellular organisms that lack a classical apoptotic machinery, it is still unclear if Bax alone is sufficient for this, or whether additional mitochondrial components are required. The protozoan parasite Giardia lamblia...
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Mémoire de diplôme HES : Haute Ecole d'Ingénierie, 2006.
Objectif : Les objectifs de ce travail de diplôme sont la production de spores de B. atrophaeus, B. coagulans et B. cereus ; la détermination de la résistance à la chaleur par méthode capillaire des spores déjà à disposition et celles nouvellement fabriquées ; la comparaison des résistances à la chaleur des spores ; l’application des succédanés de B. cereus dans la...
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Mémoire de diplôme HES : Haute Ecole d'Ingénierie, 2007.
Die Ziele der Diplomarbeit waren, Optimieren der Analytiken Methoden, Test und Beurteilung von Isolierungsmethoden, Optimierung der Aufarbeitungssequenz mit Bestimmung der Ausbeute und des reinheitsgrades. Diese konnten nicht ganz erreicht werden, da zuerst eine Optimierung der Fermentation des Organismuses in Bezug auf die Bildung des Produktes Endoinulinase erfolgen musste. Der erste...
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