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Université de Fribourg

Functional characterization of a miniature inverted transposable element at the origin of mcr-5 gene acquisition in Escherichia coli

Kieffer, Nicolas ; Nordmann, Patrice ; Millemann, Yves ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2019/63/7/e00559-19, /aac/63/7/AAC.00559-19.atom

Plasmid-mediated colistin resistance of the mobile colistin resistance (MCR) type is a growing concern in Enterobacteriaceae since it has been described worldwide in humans and animals. Here, we identified a series of MCR-producing Escherichia coli isolates corresponding to two different clones (represented by isolates PS1 and PS8b) producing MCR-1 and MCR-5, respectively, obtained from pig...

Université de Fribourg

ESBLs and resistance to ceftazidime/avibactam and ceftolozane/tazobactam combinations in Escherichia coli and Pseudomonas aeruginosa

Ortiz de la Rosa, José-Manuel ; Nordmann, Patrice ; Poirel, Laurent

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 7, p. 1934–1939

To evaluate the efficacy of the recently launched β-lactam/β-lactamase inhibitor combinations ceftazidime/avibactam and ceftolozane/tazobactam against ESBL- producing Escherichia coli and Pseudomonas aeruginosa strains.Methods: A series of ESBL-encoding genes (blaTEM, blaSHV, blaCTX-M, blaVEB, blaPER, blaGES and blaBEL) was cloned and expressed in E. coli or P. aeruginosa recipient strains. ...

Université de Fribourg

Rapid detection of fosfomycin resistance in Escherichia coli

Nordmann, Patrice ; Poirel, Laurent ; Mueller, Linda

In: Journal of Clinical Microbiology, 2019, vol. 57, no. 1, p. e01531-18

The rapid fosfomycin/Escherichia coli NP test was developed to detect fosfomycin resistance in E. coli isolates. The test is based on glucose metabolization and the detection of bacterial growth in the presence of fosfomycin at 40 µg/ml. Bacterial growth is visually detectable by an orange-to-yellow color change of red phenol, a pH indicator. A total of 100 E. coli isolates, among which 22...