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Université de Fribourg

Large-scale analysis of the mycoplasma bovis genome identified non-essential, adhesion- and virulence-related genes

Josi, Christoph ; Bürki, Sibylle ; Vidal, Sara ; Dordet-Frisoni, Emilie ; Citti, Christine ; Falquet, Laurent ; Pilo, Paola

In: Frontiers in Microbiology, 2019, vol. 10, p. -

Mycoplasma bovis is an important pathogen of cattle causing bovine mycoplasmosis. Clinical manifestations are numerous, but pneumonia, mastitis, and arthritis cases are mainly reported. Currently, no efficient vaccine is available and antibiotic treatments are not always satisfactory. The design of new, efficient prophylactic and therapeutic approaches requires a better understanding of the...

Université de Fribourg

Removal of a subset of non-essential genes fully attenuates a highly virulent mycoplasma strain

Jores, Joerg ; Ma, Li ; Ssajjakambwe, Paul ; Schieck, Elise ; Liljander, Anne ; Chandran, Suchismita ; Stoffel, Michael H. ; Cippa, Valentina ; Arfi, Yonathan ; Assad-Garcia, Nacyra ; Falquet, Laurent ; Sirand-Pugnet, Pascal ; Blanchard, Alain ; Lartigue, Carole ; Posthaus, Horst ; Labroussaa, Fabien ; Vashee, Sanjay

In: Frontiers in Microbiology, 2019, vol. 10, p. -

Mycoplasmas are the smallest free-living organisms and cause a number of economically important diseases affecting humans, animals, insects, and plants. Here, we demonstrate that highly virulent Mycoplasma mycoides subspecies capri (Mmc) can be fully attenuated via targeted deletion of non-essential genes encoding, among others, potential virulence traits. Five genomic regions, representing...