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Université de Fribourg

Hydrogen bond guidance and aromatic stacking drive liquid-liquid phase separation of intrinsically disordered histidine-rich peptides

Gabryelczyk, Bartosz ; Cai, Hao ; Shi, Xiangyan ; Sun, Yue ; Swinkels, Piet J.M. ; Salentinig, Stefan ; Pervushin, Konstantin ; Miserez, Ali

In: Nature Communications, 2019, vol. 10, no. 1, p. 1–12

Liquid-liquid phase separation (LLPS) of intrinsically disordered proteins (IDPs) is involved in both intracellular membraneless organelles and extracellular tissues. Despite growing understanding of LLPS, molecular-level mechanisms behind this process are still not fully established. Here, we use histidine-rich squid beak proteins (HBPs) as model IDPs to shed light on molecular interactions...

Université de Fribourg

Multilevel regulation of the glass locus during Drosophila eye development

Fritsch, Cornelia ; Bernardo-Garcia, F. Javier ; Humberg, Tim-Henning ; Mishra, Abhishek Kumar ; Miellet, Sara ; Almeida, Silvia ; Frochaux, Michael V. ; Deplancke, Bart ; Huber, Armin ; Sprecher, Simon G.

In: PLOS Genetics, 2019, vol. 15, no. 7, p. e1008269

Development of eye tissue is initiated by a conserved set of transcription factors termed retinal determination network (RDN). In the fruit fly Drosophila melanogaster, the zinc-finger transcription factor Glass acts directly downstream of the RDN to control identity of photoreceptor as well as non-photoreceptor cells. Tight control of spatial and temporal gene expression is a critical...

Université de Fribourg

Compartmentalization of alkaline-earth metals in salen-type Cu- and Ni-complexes in solution and in the solid state

Finelli, Alba ; Hérault, Nelly ; Crochet, Aurélien ; Fromm, Katharina M.

In: ACS Omega, 2019, vol. 4, no. 6, p. 10231–10242

The precise arrangement of metal ions in type and number by a ligand represents an important challenge in biology as well as in materials science. The preorganization of different metal ions such as alkaline-earth and transition-metal ions is of particular interest for the design of catalysts or precursors of oxides. This study is based on a Ω- shaped salen-derived ligand comprising N2O2 and...