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Université de Fribourg

From zero to six double bonds: phospholipid unsaturation and organelle function

Antonny, Bruno ; Vanni, Stefano ; Shindou, Hideo ; Thierry, Ferreira

In: Trends in Cell Biology, 2015, vol. 25, no. 7, p. 427–436

Cellular phospholipids (PLs) differ by the nature of their polar heads as well as by the length and unsaturation level of their fatty acyl chains. We discuss how the ratio between saturated, monounsaturated, and polyunsaturated PLs impacts on the functions of such organelles as the endoplasmic reticulum, synaptic vesicles, and photoreceptor discs. Recent experiments and simulations suggest...

Université de Fribourg

Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation

Cerqueira Campos, Fabiana ; Dennis, Cynthia ; Alégot, Hervé ; Fritsch, Cornelia ; Isabella, Adam ; Pouchin, Pierre ; Bardot, Olivier ; Horne-Badovinac, Sally ; Mirouse, Vincent

In: Development, 2020, vol. 147, no. 7, p. dev186957

How extracellular matrix contributes to tissue morphogenesis is still an open question. In the Drosophila ovarian follicle, it has been proposed that after Fat2-dependent planar polarization of the follicle cell basal domain, oriented basement membrane (BM) fibrils and F-actin stress fibers constrain follicle growth, promoting its axial elongation. However, the relationship between BM fibrils...

Université de Fribourg

The inoculum effect of Escherichia coli expressing mcr-1 or not on colistin activity in a murine model of peritonitis

Fantin, B. ; Poujade, J. ; Grégoire, N. ; Chau, F. ; Roujansky, A. ; Kieffer, Nicolas ; Berleur, M. ; Couet, W. ; Nordmann, Patrice

In: Clinical Microbiology and Infection, 2019, vol. 25, no. 12, p. 1563.e5-1563.e8

Colistin often remains the last resort antibiotic active against carbapenemase- producing Enterobacteriaceae. However, while in vitro inoculum effect has been reported, therapeutic relevance of this phenomenon remains questioned.Methods: Ten E. coli strains were used that included the wild-type CFT073 and its transconjugant CFT073-MCR-1 and eight susceptible clinical isolates. Mice with ...