Université de Neuchâtel

Determination of Intracellular Prolyl/Glycyl Proteases in Intact Living Human Cells and Protoporphyrin IX Production as a Reporter System

Berger, Yann ; Chapuis Bernasconi, Catherine ; Schmitt, Frédéric ; Neier, Reinhard ; Juillerat-Jeanneret, Lucienne

In: Chemistry & Biology, 2005, vol. 12, no. 8, p. 867-872

The determination of enzyme activity or inhibition in intact living cells is a problem in the development of inhibitors for intracellular proteases. The production of fluorescent protoporphyrin IX (PpIX) from the nonfluorescent (N)-Gly/Pro-5-aminolevulinic acid (ALA) substrates was used to evaluate the prolyl/glycyl-specific dipeptidylpeptidase IV (DPPIV)-like and prolyloligopeptidase (POP)-like...

Université de Neuchâtel

Bafoudiosbulbins F and G, further clerodane diterpenoids from Dioscorea bulbifera L. var sativa and revised structure of Bafoudiosbulbin B

Teponno, Rémy Bertrand ; Tapondjou, Azefack Léon ; Abou-Mansour, Eliane ; Stoeckli-Evans, Helen ; Tane, Pierre ; Barboni, Luciano

In: Phytochemistry, 2008, vol. 69, no. 12, p. 2374-2379

From the bulbils of Dioscorea bulbifera L. var sativa, two clerodane diterpenoids, Bafoudiosbulbins F (1) and G (2), together with five known compounds: Bafoudiosbulbins A–C, 3,5,4′-trihydroxy-3′-methoxybibenzyl, and kaempferol were isolated. Their structures were established by spectroscopic techniques, including 1H,13C NMR, NOESY, ROESY,...