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α-Synuclein lipoprotein nanoparticles

Eichmann, Cédric ; Bibow, Stefan ; Riek, Roland

In: Nanotechnology Reviews, 2017, vol. 6, no. 1, p. 105-110

Université de Fribourg

Estimating the accuracy of the MARTINI model towards the investigation of Peripheral Protein-Membrane Interactions

Srinivasan, Sriraksha ; Zoni, Valeria ; Vanni, Stefano

In: Faraday Discussions, 2020, p. -

Peripheral membrane proteins play a major role in numerous biological processes by transiently associating with cellular membranes, often with extreme membrane specificity. Because of the short-lived nature of these interactions, molecular dynamics (MD) simulations have emerged as an appealing tool to characterize at the structural level the molecular details of the protein-membrane...

Université de Fribourg

Responsive nanofibers with embedded hierarchical lipid self-assemblies

Tien, Nguyen D. ; Maurya, Anjani K. ; Fortunato, Giuseppino ; Rottmar, Markus ; Zboray, Robert ; Erni, Rolf ; Dommann, Alex ; Rossi, René M. ; Neels, Antonia ; Sadeghpour, Amin

In: Langmuir, 2020, vol. 36, no. 40, p. 11787–11797

We introduce the design and study of a hybrid electrospun membrane with a dedicated nanoscale structural hierarchy for controlled functions in the biomedical domain. The hybrid system comprises submicrometer-sized internally self-assembled lipid nanoparticles (ISAsomes or mesosomes) embedded into the electrospun membrane with a nanofibrous polymer network. The internal structure of ISAsomes, ...

Université de Fribourg

Conserved functions of ether lipids and sphingolipids in the early secretory pathway

Jiménez-Rojo, Noemi ; Leonetti, Manuel D. ; Zoni, Valeria ; Colom, Adai ; Feng, Suihan ; Iyengar, Namrata R. ; Matile, Stefan ; Roux, Aurélien ; Vanni, Stefano ; Weissman, Jonathan S. ; Riezman, Howard

In: Current Biology, 2020, vol. 30, no. 19, p. 3775-3787.e7

Sphingolipids play important roles in physiology and cell biology, but a systematic examination of their functions is lacking. We performed a genome-wide CRISPRi screen in sphingolipid-depleted human cells and identified hypersensitive mutants in genes of membrane trafficking and lipid biosynthesis, including ether lipid synthesis. Systematic lipidomic analysis showed a coordinate regulation...

Université de Fribourg

Accurate estimation of membrane capacitance from atomistic molecular dynamics simulations of zwitterionic lipid bilayers

Ardham, Vikram Reddy ; Zoni, Valeria ; Adamowicz, Sylvain ; Campomanes, Pablo ; Vanni, Stefano

In: The Journal of Physical Chemistry B, 2020, vol. 124, no. 38, p. 8278–8286

Lipid membranes are indispensable to life, and they regulate countless cellular processes. To investigate the properties of membranes under controlled conditions, numerous reconstitution methods have been developed over the last few decades. Several of these methods result in the formation of lipid bilayers containing residual hydrophobic molecules between the two monolayers. These...

Université de Fribourg

Methyl-branched lipids promote the membrane adsorption of α-synuclein by enhancing shallow lipid-packing defects

Garten, Matthias ; Prévost, Coline ; Cadart, Clotilde ; Gautier, Romain ; Bousset, Luc ; Melki, Ronald ; Bassereau, Patricia ; Vanni, Stefano

In: Physical Chemistry Chemical Physics, 2015, vol. 17, no. 24, p. 15589–15597

Alpha-synuclein (AS) is a synaptic protein that is directly involved in Parkinson's disease due to its tendency to form protein aggregates. Since AS aggregation can be dependent on the interactions between the protein and the cell plasma membrane, elucidating the membrane binding properties of AS is of crucial importance to establish the molecular basis of AS aggregation into toxic fibrils....

Université de Fribourg

Self-assembly of glycerol monooleate with the antimicrobial peptide LL-37: a molecular dynamics study

Innocenti Malini, R. ; Zabara, M. ; Gontsarik, M. ; Maniura-Weber, K. ; Rossi, R. M. ; Spano, F. ; Salentinig, Stefan.

In: RSC Advances, 2020, vol. 10, no. 14, p. 8291–8302

Over the past decade, the rapid increase in the incidence of antibiotic-resistant bacteria has promoted research towards alternative therapeutics such as antimicrobial peptides (AMPs), but their biodegradability limits their application. Encapsulation into nanocarriers based on the self-assembly of surfactant-like lipids is emerging as a promising strategy for the improvement of AMPs'...