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Université de Fribourg

Multiple cryoinjuries modulate the efficiency of zebrafish heart regeneration

Bise, Thomas ; Sallin, Pauline ; Pfefferli, Catherine ; Jaźwińska, Anna

In: Scientific Reports, 2020, vol. 10, no. 1, p. 11551

Zebrafish can regenerate their damaged hearts throughout their lifespan. It is, however, unknown, whether regeneration remains effective when challenged with successive cycles of cardiac damage in the same animals. Here, we assessed ventricular restoration after two, three and six cryoinjuries interspaced by recovery periods. Using transgenic cell-lineage tracing analysis, we demonstrated...

Université de Fribourg

Phosphoproteomic profiling reveals a defined genetic program for osteoblastic lineage commitment of human bone marrow–derived stromal stem cells

Barrio-Hernandez, Inigo ; Jafari, Abbas ; Rigbolt, Kristoffer T.G. ; Hallenborg, Philip ; Sanchez-Quiles, Virginia ; Skovrind, Ida ; Akimov, Vyacheslav ; Kratchmarova, Irina ; Dengjel, Jörn ; Kassem, Moustapha ; Blagoev, Blagoy

In: Genome Research, 2020, vol. 30, no. 1, p. 127-137

Bone marrow–derived mesenchymal stem cells (MSCs) differentiate into osteoblasts upon stimulation by signals present in their niche. Because the global signaling cascades involved in the early phases of MSCs osteoblast (OB) differentiation are not well-defined, we used quantitative mass spectrometry to delineate changes in human MSCs proteome and phosphoproteome during the first 24 h of...